Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis
Abstract Seven high polymorphic microsatellite loci were used to determine the pedigrees in a mass spawning of Pacific oyster, Crassostrea gigas , and to estimate the genetic variability between broodstock and offspring. Parental assignment was performed on a total of 155 individuals, including 141...
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crwiley:10.1111/j.1749-7345.2009.00286.x 2024-03-31T07:52:23+00:00 Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis Li, Ronghua L, Qi Yu, Ruihai 2009 http://dx.doi.org/10.1111/j.1749-7345.2009.00286.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1749-7345.2009.00286.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-7345.2009.00286.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the World Aquaculture Society volume 40, issue 5, page 667-677 ISSN 0893-8849 1749-7345 Agronomy and Crop Science Aquatic Science journal-article 2009 crwiley https://doi.org/10.1111/j.1749-7345.2009.00286.x 2024-03-04T13:01:10Z Abstract Seven high polymorphic microsatellite loci were used to determine the pedigrees in a mass spawning of Pacific oyster, Crassostrea gigas , and to estimate the genetic variability between broodstock and offspring. Parental assignment was performed on a total of 155 individuals, including 141 offspring, 8 candidate mothers, and 6 candidate fathers. The assignment results of real offspring were generally in agreement with simulation with a success rate over 99% using only six of these loci. The allelic diversity and observed heterozygosity ( H o ) exhibited similarity between parents and offspring populations, but the expected heterozygosity ( H e ) had a significant decrease in offspring. Although all the males and females contributed to the next generation, the variances of reproductive success and unequal sex ratio resulted in a decline in effective population size ( N e = 11.42). The inbreeding rate of this small‐scale, mass spawning population was estimated at approximately 16.5% per generation. This gave us an insight that when designing breeding programs based on mass spawning for future oyster cultivation generations, the higher inbreeding and lower effective population size should be considered. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Journal of the World Aquaculture Society 40 5 667 677 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Agronomy and Crop Science Aquatic Science |
spellingShingle |
Agronomy and Crop Science Aquatic Science Li, Ronghua L, Qi Yu, Ruihai Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
topic_facet |
Agronomy and Crop Science Aquatic Science |
description |
Abstract Seven high polymorphic microsatellite loci were used to determine the pedigrees in a mass spawning of Pacific oyster, Crassostrea gigas , and to estimate the genetic variability between broodstock and offspring. Parental assignment was performed on a total of 155 individuals, including 141 offspring, 8 candidate mothers, and 6 candidate fathers. The assignment results of real offspring were generally in agreement with simulation with a success rate over 99% using only six of these loci. The allelic diversity and observed heterozygosity ( H o ) exhibited similarity between parents and offspring populations, but the expected heterozygosity ( H e ) had a significant decrease in offspring. Although all the males and females contributed to the next generation, the variances of reproductive success and unequal sex ratio resulted in a decline in effective population size ( N e = 11.42). The inbreeding rate of this small‐scale, mass spawning population was estimated at approximately 16.5% per generation. This gave us an insight that when designing breeding programs based on mass spawning for future oyster cultivation generations, the higher inbreeding and lower effective population size should be considered. |
format |
Article in Journal/Newspaper |
author |
Li, Ronghua L, Qi Yu, Ruihai |
author_facet |
Li, Ronghua L, Qi Yu, Ruihai |
author_sort |
Li, Ronghua |
title |
Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
title_short |
Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
title_full |
Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
title_fullStr |
Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
title_full_unstemmed |
Parentage Determination and Effective Population Size Estimation in Mass Spawning Pacific Oyster, Crassostrea gigas , Based on Microsatellite Analysis |
title_sort |
parentage determination and effective population size estimation in mass spawning pacific oyster, crassostrea gigas , based on microsatellite analysis |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1749-7345.2009.00286.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1749-7345.2009.00286.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-7345.2009.00286.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal of the World Aquaculture Society volume 40, issue 5, page 667-677 ISSN 0893-8849 1749-7345 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1749-7345.2009.00286.x |
container_title |
Journal of the World Aquaculture Society |
container_volume |
40 |
container_issue |
5 |
container_start_page |
667 |
op_container_end_page |
677 |
_version_ |
1795031473198202880 |