The Induction of Tetraploidy in Salmonids

• Published in: Journal of the World Aquaculture Society
• Main Authors: Myers, James M., Hershberger, William K., Iwamoto, Robert N.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1986
• Subjects: Agronomy and Crop Science; Aquatic Science; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1749-7345.1986.tb00546.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1749-7345.1986.tb00546.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-7345.1986.tb00546.x

Abstract The induction of tetraploidy was attempted in three intraspecies crosses (autotetraploid) of sal‐monid: chinook salmon ( Oncorhynchus tshawytscha ), coho salmon ( O. kisutch ) and rainbow trout ( Salmo gairdneri ), and in the interspecies hybrid (allotetraploid) coho × Atlantic salmon ( Salmo salar ). Pressure treatments of 6.2 × 10 4 kPa (9,000 psi) were applied for six minutes at intervals from 50% of the time to cleavage to beyond first cleavage. Analysis of eyed stage embryos via flow cytometry indicated two periods of tetraploid induction existed corresponding to 55–75% and 100–110% of the first cleavage interval (FCI). The effects of different pressures and the times of application were analyzed in a study with rainbow trout. Treatments of 4.8 × 10 4 kPa (7,000 psi) for eight minutes, and 5.5 and 6.2 × 10 4 kPa (8,000 and 9,000 psi) for four minutes, were applied at 65, 78, and 100% FCI. Tetraploids were found in the 5.5 and 6.2 × 10 4 kPa group treatments only when applied at 65% FCI. The results indicate that, while tetraploidy is inducible in salmonids, further experimentation is necessary to discern the appropriate time and treatment for optimal tetraploid induction in each species.