Determination of steroid hormones in whale blow: It is possible
Abstract The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography‐mass spectrometry (LC‐MS) has been developed. Blow samples were collected opportunistica...
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crwiley:10.1111/j.1748-7692.2008.00277.x 2024-06-23T07:52:33+00:00 Determination of steroid hormones in whale blow: It is possible Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, P. J. O. Nowacek, D. 2009 http://dx.doi.org/10.1111/j.1748-7692.2008.00277.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.2008.00277.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.2008.00277.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Marine Mammal Science volume 25, issue 3, page 605-618 ISSN 0824-0469 1748-7692 journal-article 2009 crwiley https://doi.org/10.1111/j.1748-7692.2008.00277.x 2024-06-04T06:35:04Z Abstract The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography‐mass spectrometry (LC‐MS) has been developed. Blow samples were collected opportunistically from free‐ranging humpback whales ( Megaptera novaeangliae ) ( n = 35) and North Atlantic right whales ( Eubalaena glacialis ) ( n = 18) using a 13‐m carbon fiber pole with a collection device. Samples were analyzed for the presence of testosterone and progesterone using a 55% isocratic gradient with LC‐MS. Testosterone was detected in four humpback whale samples and eight northern right whale samples. Progesterone was detected in seven humpback whale samples and eight northern right whale samples. This is the first documented use of lung mucosa to determine the presence of reproductive hormones in any free‐swimming cetacean and may provide a novel non‐invasive technique to quantify the hormonal condition of free‐swimming animals that spend brief periods of time at the water's surface. Article in Journal/Newspaper Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic Wiley Online Library Marine Mammal Science 25 3 605 618 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract The feasibility of using analysis of hormone content of whale blow samples to assess reproductive function is addressed. A suitable collection method and analytical technique using liquid chromatography‐mass spectrometry (LC‐MS) has been developed. Blow samples were collected opportunistically from free‐ranging humpback whales ( Megaptera novaeangliae ) ( n = 35) and North Atlantic right whales ( Eubalaena glacialis ) ( n = 18) using a 13‐m carbon fiber pole with a collection device. Samples were analyzed for the presence of testosterone and progesterone using a 55% isocratic gradient with LC‐MS. Testosterone was detected in four humpback whale samples and eight northern right whale samples. Progesterone was detected in seven humpback whale samples and eight northern right whale samples. This is the first documented use of lung mucosa to determine the presence of reproductive hormones in any free‐swimming cetacean and may provide a novel non‐invasive technique to quantify the hormonal condition of free‐swimming animals that spend brief periods of time at the water's surface. |
format |
Article in Journal/Newspaper |
author |
Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, P. J. O. Nowacek, D. |
spellingShingle |
Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, P. J. O. Nowacek, D. Determination of steroid hormones in whale blow: It is possible |
author_facet |
Hogg, C. J. Rogers, T. L. Shorter, A. Barton, K. Miller, P. J. O. Nowacek, D. |
author_sort |
Hogg, C. J. |
title |
Determination of steroid hormones in whale blow: It is possible |
title_short |
Determination of steroid hormones in whale blow: It is possible |
title_full |
Determination of steroid hormones in whale blow: It is possible |
title_fullStr |
Determination of steroid hormones in whale blow: It is possible |
title_full_unstemmed |
Determination of steroid hormones in whale blow: It is possible |
title_sort |
determination of steroid hormones in whale blow: it is possible |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1748-7692.2008.00277.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.2008.00277.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.2008.00277.x |
genre |
Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic |
genre_facet |
Eubalaena glacialis Humpback Whale Megaptera novaeangliae North Atlantic |
op_source |
Marine Mammal Science volume 25, issue 3, page 605-618 ISSN 0824-0469 1748-7692 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1748-7692.2008.00277.x |
container_title |
Marine Mammal Science |
container_volume |
25 |
container_issue |
3 |
container_start_page |
605 |
op_container_end_page |
618 |
_version_ |
1802643899073691648 |