PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA )
ABSTRACT Wild‐caught female harbor seals ( Phoca vitulina ) were classified as sexually mature or immature on the basis of standard body length (< 125 cm immature, > 125 cm mature) and plasma progesterone concentrations measured using an enzyme‐linked immunosorbent assay (ELISA), a tec...
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Wiley
1996
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Online Access: | http://dx.doi.org/10.1111/j.1748-7692.1996.tb00575.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.1996.tb00575.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.1996.tb00575.x |
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crwiley:10.1111/j.1748-7692.1996.tb00575.x 2023-12-03T10:29:05+01:00 PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) Gardiner, K. J. Boyd, I. L. Racey, P. A. Reijnders, P. J. H. Thompson, P. M. 1996 http://dx.doi.org/10.1111/j.1748-7692.1996.tb00575.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.1996.tb00575.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.1996.tb00575.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Marine Mammal Science volume 12, issue 2, page 265-273 ISSN 0824-0469 1748-7692 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1996 crwiley https://doi.org/10.1111/j.1748-7692.1996.tb00575.x 2023-11-09T14:01:59Z ABSTRACT Wild‐caught female harbor seals ( Phoca vitulina ) were classified as sexually mature or immature on the basis of standard body length (< 125 cm immature, > 125 cm mature) and plasma progesterone concentrations measured using an enzyme‐linked immunosorbent assay (ELISA), a technique usable in the field. Sexually mature females were classified as pregnant or non‐pregnant on the basis of their plasma progesterone concentrations. Of 28 wild mature female harbor seals caught in the Moray Firth, N.E. Scotland, between the end of February and the end of May, 79% had plasma progesterone concentrations greater than 60 nmol liter −1 , the lowest plasma progesterone concentration measured in one of eight females later observed with a pup, and were diagnosed as pregnant. A linear discriminant function, calculated to provide a method of distinguishing pregnant and non‐pregnant females, predicted 100% of non‐pregnant females and 95.8% of pregnant females using plasma progesterone concentration, standard length, and month of capture as parameters. Plasma progesterone concentrations were less than 30 nmol liter −1 in all mature and immature males and immature females. In mature females plasma progesterone concentrations ranged from 0‐318 nmol liter −1 . Article in Journal/Newspaper Phoca vitulina Wiley Online Library (via Crossref) Marine Mammal Science 12 2 265 273 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Gardiner, K. J. Boyd, I. L. Racey, P. A. Reijnders, P. J. H. Thompson, P. M. PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
ABSTRACT Wild‐caught female harbor seals ( Phoca vitulina ) were classified as sexually mature or immature on the basis of standard body length (< 125 cm immature, > 125 cm mature) and plasma progesterone concentrations measured using an enzyme‐linked immunosorbent assay (ELISA), a technique usable in the field. Sexually mature females were classified as pregnant or non‐pregnant on the basis of their plasma progesterone concentrations. Of 28 wild mature female harbor seals caught in the Moray Firth, N.E. Scotland, between the end of February and the end of May, 79% had plasma progesterone concentrations greater than 60 nmol liter −1 , the lowest plasma progesterone concentration measured in one of eight females later observed with a pup, and were diagnosed as pregnant. A linear discriminant function, calculated to provide a method of distinguishing pregnant and non‐pregnant females, predicted 100% of non‐pregnant females and 95.8% of pregnant females using plasma progesterone concentration, standard length, and month of capture as parameters. Plasma progesterone concentrations were less than 30 nmol liter −1 in all mature and immature males and immature females. In mature females plasma progesterone concentrations ranged from 0‐318 nmol liter −1 . |
format |
Article in Journal/Newspaper |
author |
Gardiner, K. J. Boyd, I. L. Racey, P. A. Reijnders, P. J. H. Thompson, P. M. |
author_facet |
Gardiner, K. J. Boyd, I. L. Racey, P. A. Reijnders, P. J. H. Thompson, P. M. |
author_sort |
Gardiner, K. J. |
title |
PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
title_short |
PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
title_full |
PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
title_fullStr |
PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
title_full_unstemmed |
PLASMA PROGESTERONE CONCENTRATIONS MEASURED USING AN ENZYME‐LINKED IMMUNOSORBENT ASSAY USEFUL FOR DIAGNOSING PREGNANCY IN HARBOR SEALS ( PHOCA VITULINA ) |
title_sort |
plasma progesterone concentrations measured using an enzyme‐linked immunosorbent assay useful for diagnosing pregnancy in harbor seals ( phoca vitulina ) |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.1111/j.1748-7692.1996.tb00575.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.1996.tb00575.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.1996.tb00575.x |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Marine Mammal Science volume 12, issue 2, page 265-273 ISSN 0824-0469 1748-7692 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1748-7692.1996.tb00575.x |
container_title |
Marine Mammal Science |
container_volume |
12 |
container_issue |
2 |
container_start_page |
265 |
op_container_end_page |
273 |
_version_ |
1784254219641946112 |