IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA )
A bstract Peripheral blood was collected from 13 captive seals (12 harbor seals and 1 gray seal) of various ages to study different immunological and hematological parameters. In vitro mitogenic reactivity of blood lymphocytes was measured by means of a microculture lymphocyte transformation test (L...
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crwiley:10.1111/j.1748-7692.1995.tb00287.x 2023-12-03T10:29:06+01:00 IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) Nielsen, Jens 1995 http://dx.doi.org/10.1111/j.1748-7692.1995.tb00287.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.1995.tb00287.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.1995.tb00287.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Marine Mammal Science volume 11, issue 3, page 314-323 ISSN 0824-0469 1748-7692 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1995 crwiley https://doi.org/10.1111/j.1748-7692.1995.tb00287.x 2023-11-09T14:11:59Z A bstract Peripheral blood was collected from 13 captive seals (12 harbor seals and 1 gray seal) of various ages to study different immunological and hematological parameters. In vitro mitogenic reactivity of blood lymphocytes was measured by means of a microculture lymphocyte transformation test (LTT). After stimulation with different doses of the mitogens concanavalin A (Con A) and pokeweed mitogen (PWM), all examined seals showed significant proliferative responses to each mitogen. Furthermore, mitogenic reactivity significantly decreased with animal age suggesting this parameter of seal lymphocyte function is age‐related. The present experiments support that the LTT is a suitable tool to monitor the functional capacity of seal lymphocytes. By means of the erythrocyte‐rosette (E:‐rosette) test it was demonstrated that a subpopulation of mononuclear seal bloosd leucocytes formed rosettes with sheep red blood cells (Srbc). This observation indicates that the phenomenon probably represents a marker for T lymphocytes in the seal as in several other mammalian species. Furthermore, the percentage of Srbc rosette‐forming cells decreased with the age of the animals. Total blood leucocyte counts and differential leucocyte counts were determined by light microscopy. The number of leucocytes varied considerably among individual animals. Parallel to a significant decline of the percentage of lymphocytes with age, a corresponding increase in the percentage of neutrophils was demonstrated. In all of the seals, the percentages of monocytes and eosinophils were found to be low with only minor individual variation. The LTT and the E‐rosette test are suggested as potential tools to elucidate immunological disorders in the seal. Article in Journal/Newspaper Phoca vitulina Wiley Online Library (via Crossref) Marine Mammal Science 11 3 314 323 |
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Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Nielsen, Jens IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
A bstract Peripheral blood was collected from 13 captive seals (12 harbor seals and 1 gray seal) of various ages to study different immunological and hematological parameters. In vitro mitogenic reactivity of blood lymphocytes was measured by means of a microculture lymphocyte transformation test (LTT). After stimulation with different doses of the mitogens concanavalin A (Con A) and pokeweed mitogen (PWM), all examined seals showed significant proliferative responses to each mitogen. Furthermore, mitogenic reactivity significantly decreased with animal age suggesting this parameter of seal lymphocyte function is age‐related. The present experiments support that the LTT is a suitable tool to monitor the functional capacity of seal lymphocytes. By means of the erythrocyte‐rosette (E:‐rosette) test it was demonstrated that a subpopulation of mononuclear seal bloosd leucocytes formed rosettes with sheep red blood cells (Srbc). This observation indicates that the phenomenon probably represents a marker for T lymphocytes in the seal as in several other mammalian species. Furthermore, the percentage of Srbc rosette‐forming cells decreased with the age of the animals. Total blood leucocyte counts and differential leucocyte counts were determined by light microscopy. The number of leucocytes varied considerably among individual animals. Parallel to a significant decline of the percentage of lymphocytes with age, a corresponding increase in the percentage of neutrophils was demonstrated. In all of the seals, the percentages of monocytes and eosinophils were found to be low with only minor individual variation. The LTT and the E‐rosette test are suggested as potential tools to elucidate immunological disorders in the seal. |
format |
Article in Journal/Newspaper |
author |
Nielsen, Jens |
author_facet |
Nielsen, Jens |
author_sort |
Nielsen, Jens |
title |
IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
title_short |
IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
title_full |
IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
title_fullStr |
IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
title_full_unstemmed |
IMMUNOLOGICAL AND HEMATOLOGICAL PARAMETERS IN CAPTIVE HARBOR SEALS ( PHOCA VITULINA ) |
title_sort |
immunological and hematological parameters in captive harbor seals ( phoca vitulina ) |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1111/j.1748-7692.1995.tb00287.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-7692.1995.tb00287.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-7692.1995.tb00287.x |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Marine Mammal Science volume 11, issue 3, page 314-323 ISSN 0824-0469 1748-7692 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1748-7692.1995.tb00287.x |
container_title |
Marine Mammal Science |
container_volume |
11 |
container_issue |
3 |
container_start_page |
314 |
op_container_end_page |
323 |
_version_ |
1784254236181135360 |