On the hormonal regulation of lipolysis in isolated reindeer adipocytes
LARSEN, T.S. & NILSSEN, K.J. 1985. On the hormonal regulation of lipolysis in isolated reindeer adipocytes. Acta Physiol Scand 125 , 547–552. Received 19 December 1984ac cepted 12 June 1985. ISSN 0001–6772. Department of Arctic Biology and Department of Physiology, Institute of Medical Biology,...
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crwiley:10.1111/j.1748-1716.1985.tb07754.x 2023-12-03T10:18:18+01:00 On the hormonal regulation of lipolysis in isolated reindeer adipocytes LARSEN, TERJE S. NILSSEN, KJELL J. 1985 http://dx.doi.org/10.1111/j.1748-1716.1985.tb07754.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-1716.1985.tb07754.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-1716.1985.tb07754.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Acta Physiologica Scandinavica volume 125, issue 3, page 547-552 ISSN 0001-6772 1365-201X Physiology journal-article 1985 crwiley https://doi.org/10.1111/j.1748-1716.1985.tb07754.x 2023-11-09T14:22:46Z LARSEN, T.S. & NILSSEN, K.J. 1985. On the hormonal regulation of lipolysis in isolated reindeer adipocytes. Acta Physiol Scand 125 , 547–552. Received 19 December 1984ac cepted 12 June 1985. ISSN 0001–6772. Department of Arctic Biology and Department of Physiology, Institute of Medical Biology, University of Tromsø, Norway. The effects of insulin and bovine growth hormone (bGH) on lipolysis (basal and/or adrenaline‐stimulated) were investigated in isolated adipocytes from the Norwegian reindeer. Addition of 100 nmol 1 ‐1 adrenaline to the cells increased lipolysis 20‐fold above basal lipolytic rate. Insulin (0.1 and 1 nmol l ‐1 ) depressed lipolysis significantly at submaximal adrenaline stimulation but had no effect when lipolysis was stimulated maximally. Bovine GH (25 and 100 ng ml ‐1 ) inhibited basal lipolysis during the first 2 h of incubation, whereas refractoriness to this action was induced during the third and fourth hour of incubation. These effects were not influenced by inclusion of a small amount of glucocorticoid (dexamethasone, 25 ng ml ‐1 ). Addition of 0.1 and 1 nmol 1 ‐1 insulin together with bGH (100 ng ml ‐1 ) prevented the initial antilipolytic effect of the latter and resulted in the expression of normal basal lipolytic rates. Preincubation with bGH (100 ng ml ‐1 ) for 2 h did not alter the lipolytic response of the adipocytes to adrenaline. These findings lend support to the conclusion that insulin exerts a major control of lipolysis in reindeer adipose tissue. The significance of the antilipolytic effect of bGH is difficult to evaluate since this effect was transient and was abolished during the third and fourth hour of incubation. Article in Journal/Newspaper Arctic University of Tromsø Wiley Online Library (via Crossref) Arctic Norway Tromsø Acta Physiologica Scandinavica 125 3 547 552 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Physiology |
spellingShingle |
Physiology LARSEN, TERJE S. NILSSEN, KJELL J. On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
topic_facet |
Physiology |
description |
LARSEN, T.S. & NILSSEN, K.J. 1985. On the hormonal regulation of lipolysis in isolated reindeer adipocytes. Acta Physiol Scand 125 , 547–552. Received 19 December 1984ac cepted 12 June 1985. ISSN 0001–6772. Department of Arctic Biology and Department of Physiology, Institute of Medical Biology, University of Tromsø, Norway. The effects of insulin and bovine growth hormone (bGH) on lipolysis (basal and/or adrenaline‐stimulated) were investigated in isolated adipocytes from the Norwegian reindeer. Addition of 100 nmol 1 ‐1 adrenaline to the cells increased lipolysis 20‐fold above basal lipolytic rate. Insulin (0.1 and 1 nmol l ‐1 ) depressed lipolysis significantly at submaximal adrenaline stimulation but had no effect when lipolysis was stimulated maximally. Bovine GH (25 and 100 ng ml ‐1 ) inhibited basal lipolysis during the first 2 h of incubation, whereas refractoriness to this action was induced during the third and fourth hour of incubation. These effects were not influenced by inclusion of a small amount of glucocorticoid (dexamethasone, 25 ng ml ‐1 ). Addition of 0.1 and 1 nmol 1 ‐1 insulin together with bGH (100 ng ml ‐1 ) prevented the initial antilipolytic effect of the latter and resulted in the expression of normal basal lipolytic rates. Preincubation with bGH (100 ng ml ‐1 ) for 2 h did not alter the lipolytic response of the adipocytes to adrenaline. These findings lend support to the conclusion that insulin exerts a major control of lipolysis in reindeer adipose tissue. The significance of the antilipolytic effect of bGH is difficult to evaluate since this effect was transient and was abolished during the third and fourth hour of incubation. |
format |
Article in Journal/Newspaper |
author |
LARSEN, TERJE S. NILSSEN, KJELL J. |
author_facet |
LARSEN, TERJE S. NILSSEN, KJELL J. |
author_sort |
LARSEN, TERJE S. |
title |
On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
title_short |
On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
title_full |
On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
title_fullStr |
On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
title_full_unstemmed |
On the hormonal regulation of lipolysis in isolated reindeer adipocytes |
title_sort |
on the hormonal regulation of lipolysis in isolated reindeer adipocytes |
publisher |
Wiley |
publishDate |
1985 |
url |
http://dx.doi.org/10.1111/j.1748-1716.1985.tb07754.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-1716.1985.tb07754.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-1716.1985.tb07754.x |
geographic |
Arctic Norway Tromsø |
geographic_facet |
Arctic Norway Tromsø |
genre |
Arctic University of Tromsø |
genre_facet |
Arctic University of Tromsø |
op_source |
Acta Physiologica Scandinavica volume 125, issue 3, page 547-552 ISSN 0001-6772 1365-201X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1748-1716.1985.tb07754.x |
container_title |
Acta Physiologica Scandinavica |
container_volume |
125 |
container_issue |
3 |
container_start_page |
547 |
op_container_end_page |
552 |
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1784265278809440256 |