Autoradiography on erythrokinesis and multihemoglobins in juvenile Salmo salar L. at various respiratory gas regimes

• Published in: Acta Physiologica Scandinavica
• Main Authors: Härdig, Jan, Olsson, LarsÅke, Höglund, Lars B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1978
• Subjects: Physiology; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1748-1716.1978.tb06211.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1748-1716.1978.tb06211.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1748-1716.1978.tb06211.x

Abstract The kinetics of erylhropoiesis in 5 6 month old Salmo salar L. was correlated to 4 combinations of environmental PO 2 and pH/PCO 2 . Given single doses of 55 Fe at the start of continuous flow bioassays, the incorporation was examined in circulating red blood cells (RBC) by autoradiography on blood smears. The proportions of immature, labelled immature and labelled mature erythrocytes were calculated on samples taken at intervals up to 52 days. Compared to control fish kept at a PO 2 corresponding to 90% air saturation and pH PCO 2 at 7.6/8 pH units/mmHg, oxygen depletion to 50% air saturation stimulated the proliferation of RBC stem cells and enhanced RBC maturation. Ceteris paribus , sustained hypercapnia at a PCO 2 in the respiratory water raised to 23 mmHg (pH 7.1–7.2), stimulated proliferation but did not affect the output of mature RBC. Simultaneously lowered PO 2 and raised PCO 2 to the levels mentioned above obviously stressed the fish, as the effect of lowered PO 2 per se was not manifested. Radioactivity was traced to all clecdophorelically separable protein fractions from RBC hemolysates. The relevance of blood physiological criteria for probing the fitness or well being of fish in environmental respiratory stress conditions has been discussed.