ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID
ABSTRACT The purpose of this study was to modify the fatty acid composition of seal ( Phoca groenlandica ) blubber oil by incorporation of lauric acid (12:0; a medium chain fatty acid). Of the three enzymes that were employed in the initial screening, sn ‐1,3‐regiospecific lipase from Mucor miehei (...
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crwiley:10.1111/j.1745-4522.2006.00073.x 2023-12-03T10:29:04+01:00 ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON 2007 http://dx.doi.org/10.1111/j.1745-4522.2006.00073.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.2006.00073.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.2006.00073.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Lipids volume 14, issue 1, page 78-96 ISSN 1065-7258 1745-4522 General Chemistry Food Science journal-article 2007 crwiley https://doi.org/10.1111/j.1745-4522.2006.00073.x 2023-11-09T14:16:55Z ABSTRACT The purpose of this study was to modify the fatty acid composition of seal ( Phoca groenlandica ) blubber oil by incorporation of lauric acid (12:0; a medium chain fatty acid). Of the three enzymes that were employed in the initial screening, sn ‐1,3‐regiospecific lipase from Mucor miehei (Lipozyme IM) exhibited the highest activity and was further studied for the acidolysis of seal blubber oil with 12:0. The fatty acid composition of modified seal blubber oil was different from that of the unaltered oil. The optimum substrate mole ratio of seal blubber oil to 12:0 for M. miehei lipase was 1:3. The time course of the reaction demonstrated that 29.7% lauric acid incorporation was obtained in 24 h. Stereospecific analyses by pancreatic lipase revealed that M. miehei lipase incorporated 12:0 predominantly at the sn ‐1 and sn ‐3 positions of the triacylglycerol molecules. Article in Journal/Newspaper Phoca groenlandica Wiley Online Library (via Crossref) Journal of Food Lipids 14 1 78 96 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
General Chemistry Food Science |
spellingShingle |
General Chemistry Food Science SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
topic_facet |
General Chemistry Food Science |
description |
ABSTRACT The purpose of this study was to modify the fatty acid composition of seal ( Phoca groenlandica ) blubber oil by incorporation of lauric acid (12:0; a medium chain fatty acid). Of the three enzymes that were employed in the initial screening, sn ‐1,3‐regiospecific lipase from Mucor miehei (Lipozyme IM) exhibited the highest activity and was further studied for the acidolysis of seal blubber oil with 12:0. The fatty acid composition of modified seal blubber oil was different from that of the unaltered oil. The optimum substrate mole ratio of seal blubber oil to 12:0 for M. miehei lipase was 1:3. The time course of the reaction demonstrated that 29.7% lauric acid incorporation was obtained in 24 h. Stereospecific analyses by pancreatic lipase revealed that M. miehei lipase incorporated 12:0 predominantly at the sn ‐1 and sn ‐3 positions of the triacylglycerol molecules. |
format |
Article in Journal/Newspaper |
author |
SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON |
author_facet |
SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON |
author_sort |
SENANAYAKE, S.P.J. NAMAL |
title |
ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
title_short |
ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
title_full |
ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
title_fullStr |
ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
title_full_unstemmed |
ACIDOLYSIS OF SEAL BLUBBER OIL WITH LAURIC ACID |
title_sort |
acidolysis of seal blubber oil with lauric acid |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1745-4522.2006.00073.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.2006.00073.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.2006.00073.x |
genre |
Phoca groenlandica |
genre_facet |
Phoca groenlandica |
op_source |
Journal of Food Lipids volume 14, issue 1, page 78-96 ISSN 1065-7258 1745-4522 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1745-4522.2006.00073.x |
container_title |
Journal of Food Lipids |
container_volume |
14 |
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1 |
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78 |
op_container_end_page |
96 |
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1784254184248311808 |