ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY
ABSTRACT Lipase‐catalyzed acidolysis of evening primrose oil with docosahexaenoic acid (DHA) was carried out using Novozym‐435 from Candida antarctica as the biocatalyst. Several parameters, namely the amount of enzyme ( X 1 100–200 enzyme activity units), incubation temperature ( X 2 30–60C) and re...
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crwiley:10.1111/j.1745-4522.2006.00048.x 2024-09-15T17:48:31+00:00 ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON 2006 http://dx.doi.org/10.1111/j.1745-4522.2006.00048.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.2006.00048.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.2006.00048.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Lipids volume 13, issue 3, page 235-250 ISSN 1065-7258 1745-4522 journal-article 2006 crwiley https://doi.org/10.1111/j.1745-4522.2006.00048.x 2024-06-25T04:12:55Z ABSTRACT Lipase‐catalyzed acidolysis of evening primrose oil with docosahexaenoic acid (DHA) was carried out using Novozym‐435 from Candida antarctica as the biocatalyst. Several parameters, namely the amount of enzyme ( X 1 100–200 enzyme activity units), incubation temperature ( X 2 30–60C) and reaction time ( X 3 18–30 h) were examined as independent variables of face‐centered cube design. Using response surface methodology (RSM), the optimum reaction conditions for DHA incorporation into evening primrose oil were determined and quadratic response surface was drawn from the mathematical model. The results demonstrated that the amount of enzyme, reaction temperature and reaction time significantly ( P ≤ 0.05) affected the incorporation of DHA into evening primrose oil. Optimum reaction conditions for DHA incorporation were 162 units of enzyme at 43C in 27 h. The optimum value predicted by RSM for DHA incorporation was 31.9%. Analysis of variance showed that 95% ( R 2 = 0.95) of the observed variation was explained by the polynomial model. Close agreement existed between experimental and predicted values. The positional distribution of fatty acids in DHA‐enriched evening primrose indicated that DHA was randomly distributed among the sn ‐2 and sn ‐1 + sn ‐3 positions of the DHA‐enriched evening primrose oil. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Food Lipids 13 3 235 250 |
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Wiley Online Library |
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crwiley |
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English |
description |
ABSTRACT Lipase‐catalyzed acidolysis of evening primrose oil with docosahexaenoic acid (DHA) was carried out using Novozym‐435 from Candida antarctica as the biocatalyst. Several parameters, namely the amount of enzyme ( X 1 100–200 enzyme activity units), incubation temperature ( X 2 30–60C) and reaction time ( X 3 18–30 h) were examined as independent variables of face‐centered cube design. Using response surface methodology (RSM), the optimum reaction conditions for DHA incorporation into evening primrose oil were determined and quadratic response surface was drawn from the mathematical model. The results demonstrated that the amount of enzyme, reaction temperature and reaction time significantly ( P ≤ 0.05) affected the incorporation of DHA into evening primrose oil. Optimum reaction conditions for DHA incorporation were 162 units of enzyme at 43C in 27 h. The optimum value predicted by RSM for DHA incorporation was 31.9%. Analysis of variance showed that 95% ( R 2 = 0.95) of the observed variation was explained by the polynomial model. Close agreement existed between experimental and predicted values. The positional distribution of fatty acids in DHA‐enriched evening primrose indicated that DHA was randomly distributed among the sn ‐2 and sn ‐1 + sn ‐3 positions of the DHA‐enriched evening primrose oil. |
format |
Article in Journal/Newspaper |
author |
SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON |
spellingShingle |
SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
author_facet |
SENANAYAKE, S.P.J. NAMAL SHAHIDI, FEREIDOON |
author_sort |
SENANAYAKE, S.P.J. NAMAL |
title |
ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
title_short |
ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
title_full |
ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
title_fullStr |
ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
title_full_unstemmed |
ENZYMATIC ACIDOLYSIS OF EVENING PRIMROSE OIL WITH DOCOSAHEXAENOIC ACID USING RESPONSE SURFACE METHODOLOGY |
title_sort |
enzymatic acidolysis of evening primrose oil with docosahexaenoic acid using response surface methodology |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1745-4522.2006.00048.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.2006.00048.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.2006.00048.x |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Food Lipids volume 13, issue 3, page 235-250 ISSN 1065-7258 1745-4522 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1745-4522.2006.00048.x |
container_title |
Journal of Food Lipids |
container_volume |
13 |
container_issue |
3 |
container_start_page |
235 |
op_container_end_page |
250 |
_version_ |
1810289806150729728 |