POSITIONAL DISTRIBUTION OF FATTY ACIDS ON TRIACYLGLYCEROLS OF MENHADEN ( BREVOORTIA TYRANNIS ) AND SALMON ( SALMO SALAR ) OILS

• Published in: Journal of Food Lipids
• Main Authors: NWOSU, CHIGOZIE VICTOR, BOYD, LEON C.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1997
• Subjects: General Chemistry; Food Science; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1745-4522.1997.tb00081.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.1997.tb00081.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.1997.tb00081.x

ABSTRACT Pure triacylglycerols of both menhaden and salmon oils were separated on Florisil columns. The triacylglycerols were subjected to positional distribution analysis using a modified procedure involving reductive cleavage with a Grignard reagent. Cleaved fractions were separated on thin layer chromatography (TLC) plates using hexane‐diethyl ether‐formic acid (80:20:1, v/v/v) as the mobile phase. Separated fractions were derivatized and analyzed by gas chromatographic method to determine the component fatty acids. Results showed that salmon oil contained a greater percentage of PUFA at the Sn‐2 position of the triacylglycerol as compared to menhaden oil. The PUFA of menhaden oil were randomly distributed in the triacylglycerol. In salmon oil, a greater percentage of 18:3 (n‐3), 20:4 (n‐3), 20:5 (n‐3), 22:5 (n‐3), and 22:6 (n‐3) was in the Sn‐2 position as compared to menhaden oil. However, a greater percentage of 16:4 (n‐1), 20:4 (n‐6) and 18:4 (n‐3) was located in the Sn‐2 position of menhaden oil compared to salmon oil.