POSITIONAL DISTRIBUTION OF FATTY ACIDS ON TRIACYLGLYCEROLS OF MENHADEN ( BREVOORTIA TYRANNIS ) AND SALMON ( SALMO SALAR ) OILS
ABSTRACT Pure triacylglycerols of both menhaden and salmon oils were separated on Florisil columns. The triacylglycerols were subjected to positional distribution analysis using a modified procedure involving reductive cleavage with a Grignard reagent. Cleaved fractions were separated on thin layer...
| Published in: | Journal of Food Lipids |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
1997
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1111/j.1745-4522.1997.tb00081.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1745-4522.1997.tb00081.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4522.1997.tb00081.x |
| Summary: | ABSTRACT Pure triacylglycerols of both menhaden and salmon oils were separated on Florisil columns. The triacylglycerols were subjected to positional distribution analysis using a modified procedure involving reductive cleavage with a Grignard reagent. Cleaved fractions were separated on thin layer chromatography (TLC) plates using hexane‐diethyl ether‐formic acid (80:20:1, v/v/v) as the mobile phase. Separated fractions were derivatized and analyzed by gas chromatographic method to determine the component fatty acids. Results showed that salmon oil contained a greater percentage of PUFA at the Sn‐2 position of the triacylglycerol as compared to menhaden oil. The PUFA of menhaden oil were randomly distributed in the triacylglycerol. In salmon oil, a greater percentage of 18:3 (n‐3), 20:4 (n‐3), 20:5 (n‐3), 22:5 (n‐3), and 22:6 (n‐3) was in the Sn‐2 position as compared to menhaden oil. However, a greater percentage of 16:4 (n‐1), 20:4 (n‐6) and 18:4 (n‐3) was located in the Sn‐2 position of menhaden oil compared to salmon oil. |
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