The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non‐Antarctic) provides a tool to enable ident...
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crwiley:10.1111/j.1742-4658.2009.06954.x 2023-12-03T10:12:15+01:00 The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Martí, Marcelo A. Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Darío A. Friedman, Joel M. Mazzarella, Lelio di Prisco, Guido Verde, Cinzia 2009 http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2009.06954.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2009.06954.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FEBS Journal volume 276, issue 8, page 2266-2277 ISSN 1742-464X 1742-4658 Cell Biology Molecular Biology Biochemistry journal-article 2009 crwiley https://doi.org/10.1111/j.1742-4658.2009.06954.x 2023-11-09T14:03:21Z The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non‐Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non‐Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high‐Antarctic cold‐adapted fish. This paper focuses on the structure, function and evolution of the oxygen‐transport system of Cottoperca gobio , a sub‐Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high‐Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen‐binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen‐binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high‐Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library (via Crossref) Antarctic The Antarctic FEBS Journal 276 8 2266 2277 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Cell Biology Molecular Biology Biochemistry |
spellingShingle |
Cell Biology Molecular Biology Biochemistry Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Martí, Marcelo A. Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Darío A. Friedman, Joel M. Mazzarella, Lelio di Prisco, Guido Verde, Cinzia The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
topic_facet |
Cell Biology Molecular Biology Biochemistry |
description |
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non‐Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non‐Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high‐Antarctic cold‐adapted fish. This paper focuses on the structure, function and evolution of the oxygen‐transport system of Cottoperca gobio , a sub‐Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high‐Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen‐binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen‐binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high‐Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. |
format |
Article in Journal/Newspaper |
author |
Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Martí, Marcelo A. Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Darío A. Friedman, Joel M. Mazzarella, Lelio di Prisco, Guido Verde, Cinzia |
author_facet |
Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Martí, Marcelo A. Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Darío A. Friedman, Joel M. Mazzarella, Lelio di Prisco, Guido Verde, Cinzia |
author_sort |
Giordano, Daniela |
title |
The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
title_short |
The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
title_full |
The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
title_fullStr |
The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
title_full_unstemmed |
The hemoglobins of the sub‐Antarctic fish Cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
title_sort |
hemoglobins of the sub‐antarctic fish cottoperca gobio , a phyletically basal species – oxygen‐binding equilibria, kinetics and molecular dynamics |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2009.06954.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2009.06954.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
The FEBS Journal volume 276, issue 8, page 2266-2277 ISSN 1742-464X 1742-4658 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1742-4658.2009.06954.x |
container_title |
FEBS Journal |
container_volume |
276 |
container_issue |
8 |
container_start_page |
2266 |
op_container_end_page |
2277 |
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1784258808232542208 |