Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon
The importance of fish in vertebrate evolution has been better recognized in recent years after the intense work carried out on fish genomics. The recent discovery that fish genomes comprise homologs of ribonucleases, studied before only in tetrapods, and the isolation of ribonucleases from zebrafis...
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crwiley:10.1111/j.1742-4658.2008.06289.x 2024-06-23T07:51:19+00:00 Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon Pizzo, Elio Varcamonti, Mario Maro, Antimo D. Zanfardino, Anna Giancola, Concetta D’Alessio, Giuseppe 2008 http://dx.doi.org/10.1111/j.1742-4658.2008.06289.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2008.06289.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2008.06289.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FEBS Journal volume 275, issue 6, page 1283-1295 ISSN 1742-464X 1742-4658 journal-article 2008 crwiley https://doi.org/10.1111/j.1742-4658.2008.06289.x 2024-06-11T04:42:25Z The importance of fish in vertebrate evolution has been better recognized in recent years after the intense work carried out on fish genomics. The recent discovery that fish genomes comprise homologs of ribonucleases, studied before only in tetrapods, and the isolation of ribonucleases from zebrafish have suggested an experimental model for studying fish and vertebrate evolution. Thus, the cDNAs encoding the RNases from the Atlantic salmon were expressed, and the recombinant RNases (Ss‐RNase‐1 and Ss‐RNase‐2) were isolated and characterized as both proteins and for their biological activities. Salmon RNases are less active than RNase A in degrading RNA, but are both sensitive to the action of the human cytosolic RNase inhibitor. The two enzymes possess both angiogenic and bactericidal activities. However, catalytically inactivated Ss‐RNases do not exert any angiogenic activity, but preserve their full bactericidal activity, which is surprisingly preserved even when the enzyme proteins are fully denatured. Analyses of the conformational stability of the two RNases has revealed that they are as stable as typical RNases of the superfamily, and Ss‐RNase‐2, the most active as an enzyme, is also the most resistant to thermal and chemical denaturation. The implications of these findings in terms of the evolution of early RNases, in particular of the physiological significance of the angiogenic and bactericidal activities of fish RNases, are analyzed and discussed. Article in Journal/Newspaper Atlantic salmon Wiley Online Library FEBS Journal 275 6 1283 1295 |
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English |
description |
The importance of fish in vertebrate evolution has been better recognized in recent years after the intense work carried out on fish genomics. The recent discovery that fish genomes comprise homologs of ribonucleases, studied before only in tetrapods, and the isolation of ribonucleases from zebrafish have suggested an experimental model for studying fish and vertebrate evolution. Thus, the cDNAs encoding the RNases from the Atlantic salmon were expressed, and the recombinant RNases (Ss‐RNase‐1 and Ss‐RNase‐2) were isolated and characterized as both proteins and for their biological activities. Salmon RNases are less active than RNase A in degrading RNA, but are both sensitive to the action of the human cytosolic RNase inhibitor. The two enzymes possess both angiogenic and bactericidal activities. However, catalytically inactivated Ss‐RNases do not exert any angiogenic activity, but preserve their full bactericidal activity, which is surprisingly preserved even when the enzyme proteins are fully denatured. Analyses of the conformational stability of the two RNases has revealed that they are as stable as typical RNases of the superfamily, and Ss‐RNase‐2, the most active as an enzyme, is also the most resistant to thermal and chemical denaturation. The implications of these findings in terms of the evolution of early RNases, in particular of the physiological significance of the angiogenic and bactericidal activities of fish RNases, are analyzed and discussed. |
format |
Article in Journal/Newspaper |
author |
Pizzo, Elio Varcamonti, Mario Maro, Antimo D. Zanfardino, Anna Giancola, Concetta D’Alessio, Giuseppe |
spellingShingle |
Pizzo, Elio Varcamonti, Mario Maro, Antimo D. Zanfardino, Anna Giancola, Concetta D’Alessio, Giuseppe Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
author_facet |
Pizzo, Elio Varcamonti, Mario Maro, Antimo D. Zanfardino, Anna Giancola, Concetta D’Alessio, Giuseppe |
author_sort |
Pizzo, Elio |
title |
Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
title_short |
Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
title_full |
Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
title_fullStr |
Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
title_full_unstemmed |
Ribonucleases with angiogenic and bactericidal activities from the Atlantic salmon |
title_sort |
ribonucleases with angiogenic and bactericidal activities from the atlantic salmon |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1111/j.1742-4658.2008.06289.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2008.06289.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2008.06289.x |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
The FEBS Journal volume 275, issue 6, page 1283-1295 ISSN 1742-464X 1742-4658 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1742-4658.2008.06289.x |
container_title |
FEBS Journal |
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275 |
container_issue |
6 |
container_start_page |
1283 |
op_container_end_page |
1295 |
_version_ |
1802642379099865088 |