Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress
Groups of oysters ( Crassostrea gigas ) were exposed to 25 °C for 24 days (controls to 13 °C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 °C above the summer seawater temperature encountered in western Brittany, France where the anima...
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crwiley:10.1111/j.1742-4658.2007.06156.x 2024-06-23T07:52:17+00:00 Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress Meistertzheim, Anne‐Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie‐Thérèse 2007 http://dx.doi.org/10.1111/j.1742-4658.2007.06156.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2007.06156.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2007.06156.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FEBS Journal volume 274, issue 24, page 6392-6402 ISSN 1742-464X 1742-4658 journal-article 2007 crwiley https://doi.org/10.1111/j.1742-4658.2007.06156.x 2024-06-13T04:22:30Z Groups of oysters ( Crassostrea gigas ) were exposed to 25 °C for 24 days (controls to 13 °C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 °C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up‐ and downregulated genes in gill and mantle tissues after 7–10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real‐time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3–7 days of exposure, followed by a decrease at 14 days, and a second, less‐pronounced increase at 17–24 days. A slow‐down in protein synthesis occurred after 24 days of thermal stress. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific FEBS Journal 274 24 6392 6402 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Groups of oysters ( Crassostrea gigas ) were exposed to 25 °C for 24 days (controls to 13 °C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 °C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up‐ and downregulated genes in gill and mantle tissues after 7–10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real‐time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3–7 days of exposure, followed by a decrease at 14 days, and a second, less‐pronounced increase at 17–24 days. A slow‐down in protein synthesis occurred after 24 days of thermal stress. |
format |
Article in Journal/Newspaper |
author |
Meistertzheim, Anne‐Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie‐Thérèse |
spellingShingle |
Meistertzheim, Anne‐Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie‐Thérèse Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
author_facet |
Meistertzheim, Anne‐Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie‐Thérèse |
author_sort |
Meistertzheim, Anne‐Leila |
title |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
title_short |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
title_full |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
title_fullStr |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
title_full_unstemmed |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigasexposed to prolonged thermal stress |
title_sort |
identification of differentially expressed genes of the pacific oyster crassostrea gigasexposed to prolonged thermal stress |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1742-4658.2007.06156.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2007.06156.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2007.06156.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
The FEBS Journal volume 274, issue 24, page 6392-6402 ISSN 1742-464X 1742-4658 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1742-4658.2007.06156.x |
container_title |
FEBS Journal |
container_volume |
274 |
container_issue |
24 |
container_start_page |
6392 |
op_container_end_page |
6402 |
_version_ |
1802643549025468416 |