Crustacean hyperglycemic and vitellogenesis‐inhibiting hormones in the lobster Homarus gammarus

• Published in: FEBS Journal
• Main Authors: Ollivaux, Céline, Vinh, Joëlle, Soyez, Daniel, Toullec, Jean‐Yves
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2006
• Subjects: Cell Biology; Molecular Biology; Biochemistry; European lobster; Homarus gammarus
• Online Access: http://dx.doi.org/10.1111/j.1742-4658.2006.05228.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2006.05228.x; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2006.05228.x

Crustacean hyperglycemic hormone (CHH) and vitellogenesis‐inhibiting hormone (VIH), produced by the X organ–sinus gland neurosecretory complex, belong to a peptide group referred to as the CHH family, which is widely distributed in arthropods. In this study, genetic variants and post‐translationally modified isoforms of CHH and VIH were characterized in the European lobster Homarus gammarus . With the use of RP‐HPLC and ELISA with specific antibodies that discriminate between stereoisomers of CHH and VIH, two groups of CHH‐immunoreactive peaks were characterized from HPLC fractions of sinus gland extract (CHH A and CHH B); each group contained two variants (CHH and d ‐Phe3CHH). In the same way, two VIH‐immunoreactive peaks (VIH and d ‐Trp4VIH) were demonstrated in HPLC fractions from sinus gland extract. The masses of these different neuropeptides were determined by FT‐ICR MS: CHH A and CHH B spectra exhibited monoisotopic ions at 8557.05 Da and 8527.04 Da, respectively, and both VIH isomers displayed an m/z value of 9129.19 Da. Two full‐length cDNAs encoding preprohomones of CHH A and CHH B and only one cDNA for VIH precursor were cloned and sequenced from X organ RNA. Comparison of CHH sequences between European lobster and other Astacoidea suggests that the most hydrophobic form appeared first during crustacean evolution.