Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions
The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific g...
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crwiley:10.1111/j.1742-4658.2005.04960.x 2024-06-23T07:52:16+00:00 Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario 2005 http://dx.doi.org/10.1111/j.1742-4658.2005.04960.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2005.04960.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2005.04960.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The FEBS Journal volume 272, issue 21, page 5635-5652 ISSN 1742-464X 1742-4658 journal-article 2005 crwiley https://doi.org/10.1111/j.1742-4658.2005.04960.x 2024-06-13T04:24:32Z The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridization method was used to identify specific hypoxia up‐ and downregulated genes, in gills, mantle and digestive gland, after 7–10 days and 24 days of exposure. This method revealed 616 different sequences corresponding to 12 major physiological functions. The expression of eight potentially regulated genes was analysed by RT‐PCR in different tissues at different sampling times over the time course of hypoxia. These genes are implicated in different physiological pathways such as respiration (carbonic anhydrase), carbohydrate metabolism (glycogen phosphorylase), lipid metabolism (delta‐9 desaturase), oxidative metabolism and the immune system (glutathione peroxidase), protein regulation (BTF3, transcription factor), nucleic acid regulation (myc homologue), metal sequestration (putative metallothionein) and stress response (heat shock protein 70). Stress proteins (metallothioneins and heat shock proteins) were also quantified. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific FEBS Journal 272 21 5635 5652 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridization method was used to identify specific hypoxia up‐ and downregulated genes, in gills, mantle and digestive gland, after 7–10 days and 24 days of exposure. This method revealed 616 different sequences corresponding to 12 major physiological functions. The expression of eight potentially regulated genes was analysed by RT‐PCR in different tissues at different sampling times over the time course of hypoxia. These genes are implicated in different physiological pathways such as respiration (carbonic anhydrase), carbohydrate metabolism (glycogen phosphorylase), lipid metabolism (delta‐9 desaturase), oxidative metabolism and the immune system (glutathione peroxidase), protein regulation (BTF3, transcription factor), nucleic acid regulation (myc homologue), metal sequestration (putative metallothionein) and stress response (heat shock protein 70). Stress proteins (metallothioneins and heat shock proteins) were also quantified. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species. |
format |
Article in Journal/Newspaper |
author |
David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario |
spellingShingle |
David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
author_facet |
David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario |
author_sort |
David, Elise |
title |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
title_short |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
title_full |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
title_fullStr |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
title_full_unstemmed |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions |
title_sort |
response of the pacific oyster crassostrea gigas to hypoxia exposure under experimental conditions |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1742-4658.2005.04960.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1742-4658.2005.04960.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2005.04960.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
The FEBS Journal volume 272, issue 21, page 5635-5652 ISSN 1742-464X 1742-4658 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1742-4658.2005.04960.x |
container_title |
FEBS Journal |
container_volume |
272 |
container_issue |
21 |
container_start_page |
5635 |
op_container_end_page |
5652 |
_version_ |
1802643515267612672 |