Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction
Abstract: The H‐4‐II E enzyme induction bioassay was used for testing both pure reference substances and extracts of wildlife samples. Polychlorinated naphthalenes were found to be as active as enzyme inducers as certain coplanar polychlorinated biphenyls (PCBs). Also a mixture of polybrominated dip...
Published in: | Pharmacology & Toxicology |
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1991
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crwiley:10.1111/j.1600-0773.1991.tb01327.x 2024-09-30T14:41:55+00:00 Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction Hanberg, Annika Stahlberg, Margareta Georgellis, Antonis de Wit, Cynthia Ahlborg, Ulf G. 1991 http://dx.doi.org/10.1111/j.1600-0773.1991.tb01327.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0773.1991.tb01327.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0773.1991.tb01327.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Pharmacology & Toxicology volume 69, issue 6, page 442-449 ISSN 0901-9928 journal-article 1991 crwiley https://doi.org/10.1111/j.1600-0773.1991.tb01327.x 2024-09-19T04:19:01Z Abstract: The H‐4‐II E enzyme induction bioassay was used for testing both pure reference substances and extracts of wildlife samples. Polychlorinated naphthalenes were found to be as active as enzyme inducers as certain coplanar polychlorinated biphenyls (PCBs). Also a mixture of polybrominated diphenyl ethers (Bromkal® 70‐5DE) was shown to induce enzyme activity. In extracts of herring, containing polychlorinated dibenzo‐p‐dioxins (PCDDs) and dibenzofurans (PCDFs), bioassay and chemically derived TCDD‐equivalents (TEQs) were nearly identical. When extracts containing other types of dioxin‐like compounds as well were tested, the bioassay TEQs for most of them agreed well with chemical TEQs calculated for PCDDs, PCDFs and non‐ortho PCBs. However, for ringed seal and whitefish, TEQs obtained from the bioassay were higher than those from the chemical analysis. Our results indicate that this bioassay is an excellent complement to chemical residue analysis and a useful tool in understanding the complex interactions of halogenated hydrocarbons. For risk assessment, such results should, however, be used most carefully as they are measured in vitro. Article in Journal/Newspaper ringed seal Wiley Online Library Pharmacology & Toxicology 69 6 442 449 |
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Wiley Online Library |
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English |
description |
Abstract: The H‐4‐II E enzyme induction bioassay was used for testing both pure reference substances and extracts of wildlife samples. Polychlorinated naphthalenes were found to be as active as enzyme inducers as certain coplanar polychlorinated biphenyls (PCBs). Also a mixture of polybrominated diphenyl ethers (Bromkal® 70‐5DE) was shown to induce enzyme activity. In extracts of herring, containing polychlorinated dibenzo‐p‐dioxins (PCDDs) and dibenzofurans (PCDFs), bioassay and chemically derived TCDD‐equivalents (TEQs) were nearly identical. When extracts containing other types of dioxin‐like compounds as well were tested, the bioassay TEQs for most of them agreed well with chemical TEQs calculated for PCDDs, PCDFs and non‐ortho PCBs. However, for ringed seal and whitefish, TEQs obtained from the bioassay were higher than those from the chemical analysis. Our results indicate that this bioassay is an excellent complement to chemical residue analysis and a useful tool in understanding the complex interactions of halogenated hydrocarbons. For risk assessment, such results should, however, be used most carefully as they are measured in vitro. |
format |
Article in Journal/Newspaper |
author |
Hanberg, Annika Stahlberg, Margareta Georgellis, Antonis de Wit, Cynthia Ahlborg, Ulf G. |
spellingShingle |
Hanberg, Annika Stahlberg, Margareta Georgellis, Antonis de Wit, Cynthia Ahlborg, Ulf G. Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
author_facet |
Hanberg, Annika Stahlberg, Margareta Georgellis, Antonis de Wit, Cynthia Ahlborg, Ulf G. |
author_sort |
Hanberg, Annika |
title |
Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
title_short |
Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
title_full |
Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
title_fullStr |
Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
title_full_unstemmed |
Swedish Dioxin Survey: Evaluation of the H‐4‐II E Bioassay for Screening Environmental Samples for Dioxin‐Like Enzyme Induction |
title_sort |
swedish dioxin survey: evaluation of the h‐4‐ii e bioassay for screening environmental samples for dioxin‐like enzyme induction |
publisher |
Wiley |
publishDate |
1991 |
url |
http://dx.doi.org/10.1111/j.1600-0773.1991.tb01327.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0773.1991.tb01327.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0773.1991.tb01327.x |
genre |
ringed seal |
genre_facet |
ringed seal |
op_source |
Pharmacology & Toxicology volume 69, issue 6, page 442-449 ISSN 0901-9928 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1600-0773.1991.tb01327.x |
container_title |
Pharmacology & Toxicology |
container_volume |
69 |
container_issue |
6 |
container_start_page |
442 |
op_container_end_page |
449 |
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1811644265016590336 |