Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line
Summary Visceral organs of ectothermic vertebrates harbour melanin‐containing leukocytes termed melanomacrophages. These cells are thought to participate in immune reactions and free‐radical trapping. In teleosts, the melanin‐producing ability of melanomacrophages has hitherto not been confirmed by...
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crwiley:10.1111/j.1600-0749.2006.00297.x 2024-06-23T07:51:23+00:00 Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line Haugarvoll, Erlend Thorsen, Jim Laane, Morten Huang, Qirong Koppang, Erling Olaf 2006 http://dx.doi.org/10.1111/j.1600-0749.2006.00297.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0749.2006.00297.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0749.2006.00297.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Pigment Cell Research volume 19, issue 3, page 214-225 ISSN 0893-5785 1600-0749 journal-article 2006 crwiley https://doi.org/10.1111/j.1600-0749.2006.00297.x 2024-06-06T04:22:19Z Summary Visceral organs of ectothermic vertebrates harbour melanin‐containing leukocytes termed melanomacrophages. These cells are thought to participate in immune reactions and free‐radical trapping. In teleosts, the melanin‐producing ability of melanomacrophages has hitherto not been confirmed by molecular techniques. Here, a leukocyte marker and the apparatus for melanosome production and transport were investigated in an Atlantic salmon ( Salmo salar ) pronephros‐derived mononuclear leukocyte (SHK‐1) cell line. The SHK‐1 cells expressed transcripts specific for a mammalian CD83 homologue, a standard surface marker for activated or differentiated dendritic cells, and dopachrome tautomerase/tyrosinase‐related protein‐2, a melanocyte specific enzyme essential for melanin production. Reduction potential of melanin or its precursors was demonstrated histochemically after prolonged cultivation. Ultrastructural investigations revealed tyrosinase and acid phosphate activity in identical organelles and BSA‐gold co‐localized with multilamellar melanosomes after 2 h internalization. Apparently, melanosomes were transported and released through periodically occurring tubules fusing with the plasma membrane. Video monitoring revealed filopodia and macropinocytosis. These results showed that the SHK‐1 cell line is capable of melanogenesis and melanosome secretion. Melanin‐producing cells in teleost pronephros may represent a distinct CD83 + leukocyte population consisting of phylogenetically relict multifunctional cells. This is the first report of a melanin‐producing leukocyte cell‐line. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Pigment Cell Research 19 3 214 225 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Visceral organs of ectothermic vertebrates harbour melanin‐containing leukocytes termed melanomacrophages. These cells are thought to participate in immune reactions and free‐radical trapping. In teleosts, the melanin‐producing ability of melanomacrophages has hitherto not been confirmed by molecular techniques. Here, a leukocyte marker and the apparatus for melanosome production and transport were investigated in an Atlantic salmon ( Salmo salar ) pronephros‐derived mononuclear leukocyte (SHK‐1) cell line. The SHK‐1 cells expressed transcripts specific for a mammalian CD83 homologue, a standard surface marker for activated or differentiated dendritic cells, and dopachrome tautomerase/tyrosinase‐related protein‐2, a melanocyte specific enzyme essential for melanin production. Reduction potential of melanin or its precursors was demonstrated histochemically after prolonged cultivation. Ultrastructural investigations revealed tyrosinase and acid phosphate activity in identical organelles and BSA‐gold co‐localized with multilamellar melanosomes after 2 h internalization. Apparently, melanosomes were transported and released through periodically occurring tubules fusing with the plasma membrane. Video monitoring revealed filopodia and macropinocytosis. These results showed that the SHK‐1 cell line is capable of melanogenesis and melanosome secretion. Melanin‐producing cells in teleost pronephros may represent a distinct CD83 + leukocyte population consisting of phylogenetically relict multifunctional cells. This is the first report of a melanin‐producing leukocyte cell‐line. |
format |
Article in Journal/Newspaper |
author |
Haugarvoll, Erlend Thorsen, Jim Laane, Morten Huang, Qirong Koppang, Erling Olaf |
spellingShingle |
Haugarvoll, Erlend Thorsen, Jim Laane, Morten Huang, Qirong Koppang, Erling Olaf Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
author_facet |
Haugarvoll, Erlend Thorsen, Jim Laane, Morten Huang, Qirong Koppang, Erling Olaf |
author_sort |
Haugarvoll, Erlend |
title |
Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
title_short |
Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
title_full |
Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
title_fullStr |
Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
title_full_unstemmed |
Melanogenesis and evidence for melanosome transport to the plasma membrane in a CD83 + teleost leukocyte cell line |
title_sort |
melanogenesis and evidence for melanosome transport to the plasma membrane in a cd83 + teleost leukocyte cell line |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1600-0749.2006.00297.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0749.2006.00297.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0749.2006.00297.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pigment Cell Research volume 19, issue 3, page 214-225 ISSN 0893-5785 1600-0749 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1600-0749.2006.00297.x |
container_title |
Pigment Cell Research |
container_volume |
19 |
container_issue |
3 |
container_start_page |
214 |
op_container_end_page |
225 |
_version_ |
1802642475163058176 |