The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp.
ABSTRACT. There are two genotypes of the diplomonad Spironucleus barkhanus . Based on sequence data from the small subunit ribosomal RNA gene the conspecificity of these two genotypes has been questioned. Therefore, we have sampled Spironucleus from 27 fish, representing 14 populations, five species...
Published in: | Journal of Eukaryotic Microbiology |
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crwiley:10.1111/j.1550-7408.2006.00144.x 2024-06-02T08:00:05+00:00 The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. JØRGENSEN, ANDERS STERUD, ERIK 2006 http://dx.doi.org/10.1111/j.1550-7408.2006.00144.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1550-7408.2006.00144.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1550-7408.2006.00144.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Eukaryotic Microbiology volume 53, issue 6, page 531-541 ISSN 1066-5234 1550-7408 journal-article 2006 crwiley https://doi.org/10.1111/j.1550-7408.2006.00144.x 2024-05-03T11:59:26Z ABSTRACT. There are two genotypes of the diplomonad Spironucleus barkhanus . Based on sequence data from the small subunit ribosomal RNA gene the conspecificity of these two genotypes has been questioned. Therefore, we have sampled Spironucleus from 27 fish, representing 14 populations, five species, and four genera. Partial nucleotide sequences from the three genes; small subunit ribosomal DNA, glutamate dehydrogenase 1 and α‐tubulin were compared. The pathogenic isolates of S. barkhanus , which causes systemic spironucleosis in Atlantic salmon, Chinook salmon, and Arctic charr, all farmed in sea water, were genetically very different from the commensal isolate found in wild freshwater populations of Arctic charr and grayling. The genetic distances between the genotypes were of the same magnitude as those separating species of Giardia . Based on these genetic and ecological data, the pathogenic genotype from farmed salmonids is described as a new species, Spironucleus salmonicida n. sp. Scanning and transmission electron microscopy showed no specific morphological or ultrastructural features distinguishing S. salmonicida n. sp. from S. barkhanus . The present study clearly demonstrates the value of applying genetics in identification of Spironucleus species. Phylogenetic analyses that included the isolates of S. salmonicida n. sp. did not change the phylogenetic relationship within the genus Spironucleus . Article in Journal/Newspaper Arctic charr Arctic Atlantic salmon Wiley Online Library Arctic Journal of Eukaryotic Microbiology 53 6 531 541 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
ABSTRACT. There are two genotypes of the diplomonad Spironucleus barkhanus . Based on sequence data from the small subunit ribosomal RNA gene the conspecificity of these two genotypes has been questioned. Therefore, we have sampled Spironucleus from 27 fish, representing 14 populations, five species, and four genera. Partial nucleotide sequences from the three genes; small subunit ribosomal DNA, glutamate dehydrogenase 1 and α‐tubulin were compared. The pathogenic isolates of S. barkhanus , which causes systemic spironucleosis in Atlantic salmon, Chinook salmon, and Arctic charr, all farmed in sea water, were genetically very different from the commensal isolate found in wild freshwater populations of Arctic charr and grayling. The genetic distances between the genotypes were of the same magnitude as those separating species of Giardia . Based on these genetic and ecological data, the pathogenic genotype from farmed salmonids is described as a new species, Spironucleus salmonicida n. sp. Scanning and transmission electron microscopy showed no specific morphological or ultrastructural features distinguishing S. salmonicida n. sp. from S. barkhanus . The present study clearly demonstrates the value of applying genetics in identification of Spironucleus species. Phylogenetic analyses that included the isolates of S. salmonicida n. sp. did not change the phylogenetic relationship within the genus Spironucleus . |
format |
Article in Journal/Newspaper |
author |
JØRGENSEN, ANDERS STERUD, ERIK |
spellingShingle |
JØRGENSEN, ANDERS STERUD, ERIK The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
author_facet |
JØRGENSEN, ANDERS STERUD, ERIK |
author_sort |
JØRGENSEN, ANDERS |
title |
The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
title_short |
The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
title_full |
The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
title_fullStr |
The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
title_full_unstemmed |
The Marine Pathogenic Genotype of Spironucleus barkhanus from Farmed Salmonids Redescribed as Spironucleus salmonicida n. sp. |
title_sort |
marine pathogenic genotype of spironucleus barkhanus from farmed salmonids redescribed as spironucleus salmonicida n. sp. |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1550-7408.2006.00144.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1550-7408.2006.00144.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1550-7408.2006.00144.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon |
genre_facet |
Arctic charr Arctic Atlantic salmon |
op_source |
Journal of Eukaryotic Microbiology volume 53, issue 6, page 531-541 ISSN 1066-5234 1550-7408 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1550-7408.2006.00144.x |
container_title |
Journal of Eukaryotic Microbiology |
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53 |
container_issue |
6 |
container_start_page |
531 |
op_container_end_page |
541 |
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1800744096882491392 |