INORGANIC CARBON UTILIZATION BY ROSS SEA PHYTOPLANKTON ACROSS NATURAL AND EXPERIMENTAL CO 2 GRADIENTS 1

• Published in: Journal of Phycology
• Main Authors: Tortell, Philippe D., Trimborn, Scarlett, Li, Yingyu, Rost, Björn, Payne, Christopher D.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2010
• Subjects: Ross Sea
• Online Access: http://dx.doi.org/10.1111/j.1529-8817.2010.00839.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2010.00839.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2010.00839.x

We present results from a field study of inorganic carbon (C) acquisition by Ross Sea phytoplankton during Phaeocystis ‐dominated early season blooms. Isotope disequilibrium experiments revealed that HCO 3 − was the primary inorganic C source for photosynthesis in all phytoplankton assemblages. From these experiments, we also derived relative enhancement factors for HCO 3 − /CO 2 interconversion as a measure of extracellular carbonic anhydrase activity (eCA). The enhancement factors ranged from 1.0 (no apparent eCA activity) to 6.4, with an overall mean of 2.9. Additional eCA measurements, made using membrane inlet mass spectrometry (MIMS), yielded activities ranging from 2.4 to 6.9 U · [μg chl a ] −1 (mean 4.1). Measurements of short‐term C‐fixation parameters revealed saturation kinetics with respect to external inorganic carbon, with a mean half‐saturation constant for inorganic carbon uptake ( K 1/2 ) of ∼380 μM. Comparison of our early springtime results with published data from late‐season Ross Sea assemblages showed that neither HCO 3 − utilization nor eCA activity was significantly correlated to ambient CO 2 levels or phytoplankton taxonomic composition. We did, however, observe a strong negative relationship between surface water pCO 2 and short‐term 14 C‐fixation rates for the early season survey. Direct incubation experiments showed no statistically significant effects of pCO 2 (10 to 80 Pa) on relative HCO 3 − utilization or eCA activity. Our results provide insight into the seasonal regulation of C uptake by Ross Sea phytoplankton across a range of pCO 2 and phytoplankton taxonomic composition.