SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1
Environmental PCR is a common tool for surveying aquatic microalgae; however, universal primers generally employed are not specific to phytoplankton and typically recover nonphotosynthetic bacteria at high frequencies. Using a 16S rDNA “phyto‐specific” primer, we were able to selectively amplify seq...
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crwiley:10.1111/j.1529-8817.2007.00350.x 2024-06-23T07:56:42+00:00 SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 Betournay, Scott Marsh, Amanda C. Donello, Nicholas Stiller, John W. 2007 http://dx.doi.org/10.1111/j.1529-8817.2007.00350.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2007.00350.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2007.00350.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 43, issue 3, page 609-613 ISSN 0022-3646 1529-8817 journal-article 2007 crwiley https://doi.org/10.1111/j.1529-8817.2007.00350.x 2024-06-11T04:46:20Z Environmental PCR is a common tool for surveying aquatic microalgae; however, universal primers generally employed are not specific to phytoplankton and typically recover nonphotosynthetic bacteria at high frequencies. Using a 16S rDNA “phyto‐specific” primer, we were able to selectively amplify sequences of photosynthetic species from several mixed aquatic samples, even when large numbers of nonphotosynthetic microorganisms were present. We identified 21 microalgal sequences from three different habitats: salt marshes in Virginia, river basins in North Carolina, and sea ice in Alaska. In contrast, universal 16S primers recovered a majority of nonphotosynthetic organisms from some of the same samples. Our results indicate that phytoplankton‐specific primers are efficient in selectively amplifying a broad diversity of microalgae in mixed environmental samples and, therefore, can reduce the noise from extraneous species that often dominates molecular surveys of aquatic samples. Article in Journal/Newspaper Sea ice Alaska Wiley Online Library Journal of Phycology 43 3 609 613 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Environmental PCR is a common tool for surveying aquatic microalgae; however, universal primers generally employed are not specific to phytoplankton and typically recover nonphotosynthetic bacteria at high frequencies. Using a 16S rDNA “phyto‐specific” primer, we were able to selectively amplify sequences of photosynthetic species from several mixed aquatic samples, even when large numbers of nonphotosynthetic microorganisms were present. We identified 21 microalgal sequences from three different habitats: salt marshes in Virginia, river basins in North Carolina, and sea ice in Alaska. In contrast, universal 16S primers recovered a majority of nonphotosynthetic organisms from some of the same samples. Our results indicate that phytoplankton‐specific primers are efficient in selectively amplifying a broad diversity of microalgae in mixed environmental samples and, therefore, can reduce the noise from extraneous species that often dominates molecular surveys of aquatic samples. |
format |
Article in Journal/Newspaper |
author |
Betournay, Scott Marsh, Amanda C. Donello, Nicholas Stiller, John W. |
spellingShingle |
Betournay, Scott Marsh, Amanda C. Donello, Nicholas Stiller, John W. SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
author_facet |
Betournay, Scott Marsh, Amanda C. Donello, Nicholas Stiller, John W. |
author_sort |
Betournay, Scott |
title |
SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
title_short |
SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
title_full |
SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
title_fullStr |
SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
title_full_unstemmed |
SELECTIVE RECOVERY OF MICROALGAE FROM DIVERSE HABITATS USING “PHYTO‐SPECIFIC” 16S rDNA PRIMERS 1 |
title_sort |
selective recovery of microalgae from diverse habitats using “phyto‐specific” 16s rdna primers 1 |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1529-8817.2007.00350.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2007.00350.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2007.00350.x |
genre |
Sea ice Alaska |
genre_facet |
Sea ice Alaska |
op_source |
Journal of Phycology volume 43, issue 3, page 609-613 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1529-8817.2007.00350.x |
container_title |
Journal of Phycology |
container_volume |
43 |
container_issue |
3 |
container_start_page |
609 |
op_container_end_page |
613 |
_version_ |
1802649998359265280 |