INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1
Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was condu...
Published in: | Journal of Phycology |
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crwiley:10.1111/j.1529-8817.2006.00231.x 2024-09-15T18:24:44+00:00 INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 Débora Iglesias‐Rodríguez, María Schofield, Oscar M. Batley, Jacqueline Medlin, Linda K. Hayes, Paul K. 2006 http://dx.doi.org/10.1111/j.1529-8817.2006.00231.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2006.00231.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2006.00231.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 42, issue 3, page 526-536 ISSN 0022-3646 1529-8817 journal-article 2006 crwiley https://doi.org/10.1111/j.1529-8817.2006.00231.x 2024-08-01T04:20:31Z Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) ( n =41) and a North East Atlantic (NEA) group ( n =21), using F ST as an indicator of genetic differentiation, revealed moderate genetic differentiation ( F ST =0.09894; P =0; significance level=0.05). Gene flow between the FJ92 and NEA groups was estimated to be low, which is in agreement with the moderate levels of genetic differentiation revealed by the microsatellite data. A genetic assignment method that uses genotype likelihoods to draw inference about the groups to which individuals belong was tested. Using FJ92 and NEA as reference groups, we observed that all the E. huxleyi groups tested against the two reference groups were unrelated to them. On a global biogeographical scale, E. huxleyi populations appear to be highly genetically diverse. Our findings raise the question of whether such a high degree of intraspecific genetic diversity in coccolithophores translates into variability in ecological function. Article in Journal/Newspaper North East Atlantic Wiley Online Library Journal of Phycology 42 3 526 536 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Using primer pairs for seven previously described microsatellite loci and three newly characterized microsatellite loci from the coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler, we assessed genetic variation within this species. Analysis of microsatellite length variants (alleles) was conducted for 85 E. huxleyi isolates representative of different ocean basins. These results revealed high intraspecific genetic variability within the E. huxleyi species concept. Pairwise comparison of a 1992 Coastal Fjord group (FJ92) ( n =41) and a North East Atlantic (NEA) group ( n =21), using F ST as an indicator of genetic differentiation, revealed moderate genetic differentiation ( F ST =0.09894; P =0; significance level=0.05). Gene flow between the FJ92 and NEA groups was estimated to be low, which is in agreement with the moderate levels of genetic differentiation revealed by the microsatellite data. A genetic assignment method that uses genotype likelihoods to draw inference about the groups to which individuals belong was tested. Using FJ92 and NEA as reference groups, we observed that all the E. huxleyi groups tested against the two reference groups were unrelated to them. On a global biogeographical scale, E. huxleyi populations appear to be highly genetically diverse. Our findings raise the question of whether such a high degree of intraspecific genetic diversity in coccolithophores translates into variability in ecological function. |
format |
Article in Journal/Newspaper |
author |
Débora Iglesias‐Rodríguez, María Schofield, Oscar M. Batley, Jacqueline Medlin, Linda K. Hayes, Paul K. |
spellingShingle |
Débora Iglesias‐Rodríguez, María Schofield, Oscar M. Batley, Jacqueline Medlin, Linda K. Hayes, Paul K. INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
author_facet |
Débora Iglesias‐Rodríguez, María Schofield, Oscar M. Batley, Jacqueline Medlin, Linda K. Hayes, Paul K. |
author_sort |
Débora Iglesias‐Rodríguez, María |
title |
INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
title_short |
INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
title_full |
INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
title_fullStr |
INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
title_full_unstemmed |
INTRASPECIFIC GENETIC DIVERSITY IN THE MARINE COCCOLITHOPHORE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE): THE USE OF MICROSATELLITE ANALYSIS IN MARINE PHYTOPLANKTON POPULATION STUDIES 1 |
title_sort |
intraspecific genetic diversity in the marine coccolithophore emiliania huxleyi (prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies 1 |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1529-8817.2006.00231.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2006.00231.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2006.00231.x |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_source |
Journal of Phycology volume 42, issue 3, page 526-536 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1529-8817.2006.00231.x |
container_title |
Journal of Phycology |
container_volume |
42 |
container_issue |
3 |
container_start_page |
526 |
op_container_end_page |
536 |
_version_ |
1810465147528937472 |