ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1
The biodiversity of protistan assemblages present in microhabitats of the Ross Sea, Antarctica, was examined using molecular biological methods to obtain a greater understanding of the genetic diversity present. Sequencing of 18S clone libraries indicated genetically diverse collections of organisms...
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crwiley:10.1111/j.1529-8817.2006.00183.x 2024-09-15T17:47:47+00:00 ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 Gast, Rebecca J. Moran, Dawn M. Beaudoin, David J. Blythe, Jonathan N. Dennett, Mark R. Caron, David A. 2006 http://dx.doi.org/10.1111/j.1529-8817.2006.00183.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2006.00183.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2006.00183.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 42, issue 1, page 233-242 ISSN 0022-3646 1529-8817 journal-article 2006 crwiley https://doi.org/10.1111/j.1529-8817.2006.00183.x 2024-07-25T04:22:53Z The biodiversity of protistan assemblages present in microhabitats of the Ross Sea, Antarctica, was examined using molecular biological methods to obtain a greater understanding of the genetic diversity present. Sequencing of 18S clone libraries indicated genetically diverse collections of organisms in the water column, ice, and meltwater layer (slush), but a single small subunit ribosomal DNA (srDNA) sequence type dominated clone libraries (>30%) from seawater and slush samples taken within the ice pack of this ecosystem. The BLAST searches indicated that this dominant clone was derived from a dinoflagellate, and that it shared sequence similarity (97.6%–98.3%) with both Karenia and Karlodinium species. Phylogenetic analyses based on small subunit ribosomal gene sequences supported its placement as a sister group to these taxa, and suggested that it represented a novel genus. The dinoflagellate was successfully recovered in culture, and morphological analyses have shown that it contains chloroplasts, is gymnodinoid, appears not to have thecal plates, and has an apical groove and sulcal structure that confirm its placement as a relative of the Karenia/Karlodinium group. The abundance of this phylotype in natural samples was confirmed by quantitative PCR analyses of water and slush communities, and suggests that this dinoflagellate can be a major constituent of the protistan assemblages of some Antarctic microhabitats of the Ross Sea. Article in Journal/Newspaper Antarc* Antarctic Antarctica ice pack Ross Sea Wiley Online Library Journal of Phycology 42 1 233 242 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The biodiversity of protistan assemblages present in microhabitats of the Ross Sea, Antarctica, was examined using molecular biological methods to obtain a greater understanding of the genetic diversity present. Sequencing of 18S clone libraries indicated genetically diverse collections of organisms in the water column, ice, and meltwater layer (slush), but a single small subunit ribosomal DNA (srDNA) sequence type dominated clone libraries (>30%) from seawater and slush samples taken within the ice pack of this ecosystem. The BLAST searches indicated that this dominant clone was derived from a dinoflagellate, and that it shared sequence similarity (97.6%–98.3%) with both Karenia and Karlodinium species. Phylogenetic analyses based on small subunit ribosomal gene sequences supported its placement as a sister group to these taxa, and suggested that it represented a novel genus. The dinoflagellate was successfully recovered in culture, and morphological analyses have shown that it contains chloroplasts, is gymnodinoid, appears not to have thecal plates, and has an apical groove and sulcal structure that confirm its placement as a relative of the Karenia/Karlodinium group. The abundance of this phylotype in natural samples was confirmed by quantitative PCR analyses of water and slush communities, and suggests that this dinoflagellate can be a major constituent of the protistan assemblages of some Antarctic microhabitats of the Ross Sea. |
format |
Article in Journal/Newspaper |
author |
Gast, Rebecca J. Moran, Dawn M. Beaudoin, David J. Blythe, Jonathan N. Dennett, Mark R. Caron, David A. |
spellingShingle |
Gast, Rebecca J. Moran, Dawn M. Beaudoin, David J. Blythe, Jonathan N. Dennett, Mark R. Caron, David A. ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
author_facet |
Gast, Rebecca J. Moran, Dawn M. Beaudoin, David J. Blythe, Jonathan N. Dennett, Mark R. Caron, David A. |
author_sort |
Gast, Rebecca J. |
title |
ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
title_short |
ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
title_full |
ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
title_fullStr |
ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
title_full_unstemmed |
ABUNDANCE OF A NOVEL DINOFLAGELLATE PHYLOTYPE IN THE ROSS SEA, ANTARCTICA 1 |
title_sort |
abundance of a novel dinoflagellate phylotype in the ross sea, antarctica 1 |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1529-8817.2006.00183.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2006.00183.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2006.00183.x |
genre |
Antarc* Antarctic Antarctica ice pack Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica ice pack Ross Sea |
op_source |
Journal of Phycology volume 42, issue 1, page 233-242 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1529-8817.2006.00183.x |
container_title |
Journal of Phycology |
container_volume |
42 |
container_issue |
1 |
container_start_page |
233 |
op_container_end_page |
242 |
_version_ |
1810497369485082624 |