ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1
Polarella glacialis (Montresor et al.) was identified in Davis Station sea ice by morphological and DNA sequence comparison of cultures with those of the authentic strain P. glacialis CCMP 1383 isolated from McMurdo Sound. Cells and cysts of the Davis isolate (FL1B) were morphologically indistinguis...
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crwiley:10.1111/j.1529-8817.2004.03169.x 2024-06-23T07:47:27+00:00 ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 Thomson, Paul G. Wright, Simon W. Bolch, Christopher J. S. Nichols, Peter D. Skerratt, Jennifer H. McMinn, Andrew 2004 http://dx.doi.org/10.1111/j.1529-8817.2004.03169.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2004.03169.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2004.03169.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 40, issue 5, page 867-873 ISSN 0022-3646 1529-8817 journal-article 2004 crwiley https://doi.org/10.1111/j.1529-8817.2004.03169.x 2024-06-13T04:21:15Z Polarella glacialis (Montresor et al.) was identified in Davis Station sea ice by morphological and DNA sequence comparison of cultures with those of the authentic strain P. glacialis CCMP 1383 isolated from McMurdo Sound. Cells and cysts of the Davis isolate (FL1B) were morphologically indistinguishable from P. glacialis , and comparison of the large subunit rDNA of both cultures demonstrated only 0.2% sequence divergence over 1366 base pairs. The photosynthetic pigments of P. glacialis (strains FL1B and CCMP 1383) were typical of dinoflagellates, with peridinin (contributing up to 31%) as the major accessory pigment. Extremely high levels of polyunsaturated fatty acids (PUFA, up to 76.3%) were characteristic of P. glacialis isolate FL1B. The high PUFA concentration of this species is thought to be an adaptation to survive the cold temperatures of the upper fast ice. The sterol profile of FL1B was atypical of dinoflagellates, with 4‐desmethylsterols (up to 79%) in greater abundance than 4α‐methyl sterols (up to 24%). 27‐ Nor ‐24‐methylcholest‐5,22E‐dien‐3β‐ol was identified as the principle sterol in P. glacialis , contributing up to 64% of the total sterol composition. Article in Journal/Newspaper Antarc* Antarctic McMurdo Sound Sea ice Wiley Online Library Antarctic Davis Station ENVELOPE(77.968,77.968,-68.576,-68.576) Davis-Station ENVELOPE(77.968,77.968,-68.576,-68.576) McMurdo Sound Journal of Phycology 40 5 867 873 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Polarella glacialis (Montresor et al.) was identified in Davis Station sea ice by morphological and DNA sequence comparison of cultures with those of the authentic strain P. glacialis CCMP 1383 isolated from McMurdo Sound. Cells and cysts of the Davis isolate (FL1B) were morphologically indistinguishable from P. glacialis , and comparison of the large subunit rDNA of both cultures demonstrated only 0.2% sequence divergence over 1366 base pairs. The photosynthetic pigments of P. glacialis (strains FL1B and CCMP 1383) were typical of dinoflagellates, with peridinin (contributing up to 31%) as the major accessory pigment. Extremely high levels of polyunsaturated fatty acids (PUFA, up to 76.3%) were characteristic of P. glacialis isolate FL1B. The high PUFA concentration of this species is thought to be an adaptation to survive the cold temperatures of the upper fast ice. The sterol profile of FL1B was atypical of dinoflagellates, with 4‐desmethylsterols (up to 79%) in greater abundance than 4α‐methyl sterols (up to 24%). 27‐ Nor ‐24‐methylcholest‐5,22E‐dien‐3β‐ol was identified as the principle sterol in P. glacialis , contributing up to 64% of the total sterol composition. |
format |
Article in Journal/Newspaper |
author |
Thomson, Paul G. Wright, Simon W. Bolch, Christopher J. S. Nichols, Peter D. Skerratt, Jennifer H. McMinn, Andrew |
spellingShingle |
Thomson, Paul G. Wright, Simon W. Bolch, Christopher J. S. Nichols, Peter D. Skerratt, Jennifer H. McMinn, Andrew ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
author_facet |
Thomson, Paul G. Wright, Simon W. Bolch, Christopher J. S. Nichols, Peter D. Skerratt, Jennifer H. McMinn, Andrew |
author_sort |
Thomson, Paul G. |
title |
ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
title_short |
ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
title_full |
ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
title_fullStr |
ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
title_full_unstemmed |
ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1 |
title_sort |
antarctic distribution, pigment and lipid composition, and molecular identification of the brine dinoflagellate polarella glacialis (dinophyceae) 1 |
publisher |
Wiley |
publishDate |
2004 |
url |
http://dx.doi.org/10.1111/j.1529-8817.2004.03169.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.2004.03169.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.2004.03169.x |
long_lat |
ENVELOPE(77.968,77.968,-68.576,-68.576) ENVELOPE(77.968,77.968,-68.576,-68.576) |
geographic |
Antarctic Davis Station Davis-Station McMurdo Sound |
geographic_facet |
Antarctic Davis Station Davis-Station McMurdo Sound |
genre |
Antarc* Antarctic McMurdo Sound Sea ice |
genre_facet |
Antarc* Antarctic McMurdo Sound Sea ice |
op_source |
Journal of Phycology volume 40, issue 5, page 867-873 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1529-8817.2004.03169.x |
container_title |
Journal of Phycology |
container_volume |
40 |
container_issue |
5 |
container_start_page |
867 |
op_container_end_page |
873 |
_version_ |
1802651562412081152 |