KINETICS OF AMINO ACID INFLUX INTO NITELLA FLEXILIS 1

• Published in: Journal of Phycology
• Main Author: Wallen, D. G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1973
• Subjects: Nitella flexilis
• Online Access: http://dx.doi.org/10.1111/j.1529-8817.1973.tb04070.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1529-8817.1973.tb04070.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1529-8817.1973.tb04070.x

SUMMARY The uptake of amino acids by Nitella flexilis has been investigated. Influx of glycine, alanine, and valine appears to be a diffusive process. Influx ranged from 0.14 to 0.06 and 0.04 pmoles/(cm)(sec), respectively. Aspartic acid uptake is an active transport mechanism. The V max is 2.8 pmoles/(cm)(sec); the transport constant (Michaelis constant) K m , 7.8 × 10 −3 M. The uptake of arginine is apparently due to 2 transport systems, one with a V max and K m of 3.1 pmoles/(cm)(sec) and 3.2 × 10 −3 M, respectively. The second system has a V max of 1.4 pmoles/(cm)(sec) and a K m of 2.1 × 10 −4 M. The possibility that the second system is diffusive has been considered.