EST‐SSR markers from the Pacific oyster, Crassostrea gigas

• Published in: Molecular Ecology Notes
• Main Authors: YU, HONG, LI, QI
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2007
• Subjects: Crassostrea gigas; Pacific oyster
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2007.01729.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01729.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01729.x

Abstract Ten polymorphic microsatellite repeat markers were identified from Crassostrea gigas, expressed sequence tags (EST) deposited in public sequence database. Number of alleles per locus ranged from three to 18, expected and observed heterozygosities ranged from 0.071 to 0.738 and from 0.306 to 0.913, respectively. Marker transferability was tested on other two Crassostrea species and polymorphic products were detected at nine loci. EST‐derived simple sequence repeats provide robust, informative and potentially transferable polymorphic markers suitable for population genetic, parentage, and mapping studies of C. gigas .