Isolation and characterization of polymorphic microsatellite loci from an EST library of turbot ( Scophthalmus maximus) and cross‐species amplification

Abstract In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus maximus . Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs o...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: CHEN, S. L., MA, H. Y., JIANG, Y., LIAO, X. L., MENG, L.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2007
Subjects:
Scophthalmus maximus
Turbot
Online Access:http://dx.doi.org/10.1111/j.1471-8286.2007.01725.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01725.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01725.x
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Summary:Abstract In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus maximus . Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Cross‐species amplifications of these microsatellites in five additional fish species revealed between five and 11 positive amplifications and between zero and four polymorphic loci per species.

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