Characterization of tetranucleotide microsatellite loci and development and validation of multiplex reactions for the study of right whale species (genus Eubalaena)

• Published in: Molecular Ecology Notes
• Main Authors: FRASIER, T. R., RASTOGI, T., BROWN, M. W., HAMILTON, P. K., KRAUS, S. D., WHITE, B. N.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2006
• Subjects: Gata; Eubalaena glacialis; North Atlantic; North Atlantic right whale
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2006.01417.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2006.01417.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2006.01417.x

Abstract A North Atlantic right whale ( Eubalaena glacialis ) genomic library was developed and screened with a (GATA) 8 probe to identify tetranucleotide microsatellite loci. Sixteen characterized loci were polymorphic in North Atlantic and/or South Atlantic ( Eubalaena australis ) right whales, 12 being polymorphic in E. glacialis , and 15 in E. australis . Fourteen of these were combined with 21 other previously identified loci for a suite of 35 loci which can be used to increase resolution of genetic analyses of these species. Multiplex reactions were developed for genotyping samples at these loci, providing a method that is rapid, reliable and cost‐effective.