Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification
Abstract Five polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in turbot, Scophthalmus maximus . Twelve microsatellites were selected for designing microsatellite primers, of which five gave working primer pairs. They had between four...
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crwiley:10.1111/j.1471-8286.2006.01379.x 2024-06-02T08:14:09+00:00 Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification LIU, Y. G. LIU, L. X. LEI, Z. W. GAO, A. Y. LI, B. F. 2006 http://dx.doi.org/10.1111/j.1471-8286.2006.01379.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2006.01379.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2006.01379.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Notes volume 6, issue 3, page 867-869 ISSN 1471-8278 1471-8286 journal-article 2006 crwiley https://doi.org/10.1111/j.1471-8286.2006.01379.x 2024-05-03T11:19:26Z Abstract Five polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in turbot, Scophthalmus maximus . Twelve microsatellites were selected for designing microsatellite primers, of which five gave working primer pairs. They had between four and nine alleles. Observed and expected heterozygosities varied from 0.76 to 0.90 and from 0.63 to 0.83, respectively. Five additional fish species assessed for cross‐species amplification revealed between one and three positive amplifications and between zero and two polymorphic loci per species. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Molecular Ecology Notes 6 3 867 869 |
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Wiley Online Library |
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English |
description |
Abstract Five polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in turbot, Scophthalmus maximus . Twelve microsatellites were selected for designing microsatellite primers, of which five gave working primer pairs. They had between four and nine alleles. Observed and expected heterozygosities varied from 0.76 to 0.90 and from 0.63 to 0.83, respectively. Five additional fish species assessed for cross‐species amplification revealed between one and three positive amplifications and between zero and two polymorphic loci per species. |
format |
Article in Journal/Newspaper |
author |
LIU, Y. G. LIU, L. X. LEI, Z. W. GAO, A. Y. LI, B. F. |
spellingShingle |
LIU, Y. G. LIU, L. X. LEI, Z. W. GAO, A. Y. LI, B. F. Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
author_facet |
LIU, Y. G. LIU, L. X. LEI, Z. W. GAO, A. Y. LI, B. F. |
author_sort |
LIU, Y. G. |
title |
Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
title_short |
Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
title_full |
Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
title_fullStr |
Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
title_full_unstemmed |
Identification of polymorphic microsatellite markers from RAPD product in turbot ( Scophthalmus maximus) and a test of cross‐species amplification |
title_sort |
identification of polymorphic microsatellite markers from rapd product in turbot ( scophthalmus maximus) and a test of cross‐species amplification |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1471-8286.2006.01379.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2006.01379.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2006.01379.x |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Molecular Ecology Notes volume 6, issue 3, page 867-869 ISSN 1471-8278 1471-8286 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1471-8286.2006.01379.x |
container_title |
Molecular Ecology Notes |
container_volume |
6 |
container_issue |
3 |
container_start_page |
867 |
op_container_end_page |
869 |
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1800737880692228096 |