Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae)
Abstract We report the development of 14 novel polymorphic microsatellite markers cloned from the White‐tailed Sea Eagle, Haliaeetus albicilla , a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain re...
Published in: | Molecular Ecology Notes |
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crwiley:10.1111/j.1471-8286.2005.01122.x 2024-06-02T08:07:44+00:00 Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) HAILER, FRANK GAUTSCHI, BARBARA HELANDER, BJÖRN 2005 http://dx.doi.org/10.1111/j.1471-8286.2005.01122.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01122.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01122.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Notes volume 5, issue 4, page 938-940 ISSN 1471-8278 1471-8286 journal-article 2005 crwiley https://doi.org/10.1111/j.1471-8286.2005.01122.x 2024-05-03T12:07:38Z Abstract We report the development of 14 novel polymorphic microsatellite markers cloned from the White‐tailed Sea Eagle, Haliaeetus albicilla , a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla , but which may be of interest for use in other raptor species. Article in Journal/Newspaper Haliaeetus albicilla Wiley Online Library Molecular Ecology Notes 5 4 938 940 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract We report the development of 14 novel polymorphic microsatellite markers cloned from the White‐tailed Sea Eagle, Haliaeetus albicilla , a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla , but which may be of interest for use in other raptor species. |
format |
Article in Journal/Newspaper |
author |
HAILER, FRANK GAUTSCHI, BARBARA HELANDER, BJÖRN |
spellingShingle |
HAILER, FRANK GAUTSCHI, BARBARA HELANDER, BJÖRN Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
author_facet |
HAILER, FRANK GAUTSCHI, BARBARA HELANDER, BJÖRN |
author_sort |
HAILER, FRANK |
title |
Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
title_short |
Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
title_full |
Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
title_fullStr |
Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
title_full_unstemmed |
Development and multiplex PCR amplification of novel microsatellite markers in the White‐tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae) |
title_sort |
development and multiplex pcr amplification of novel microsatellite markers in the white‐tailed sea eagle, haliaeetus albicilla (aves: falconiformes, accipitridae) |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1471-8286.2005.01122.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01122.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01122.x |
genre |
Haliaeetus albicilla |
genre_facet |
Haliaeetus albicilla |
op_source |
Molecular Ecology Notes volume 5, issue 4, page 938-940 ISSN 1471-8278 1471-8286 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1471-8286.2005.01122.x |
container_title |
Molecular Ecology Notes |
container_volume |
5 |
container_issue |
4 |
container_start_page |
938 |
op_container_end_page |
940 |
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1800752854185541632 |