Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags
Abstract The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST databa...
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crwiley:10.1111/j.1471-8286.2005.01056.x 2024-09-15T17:55:45+00:00 Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags NG, SIEMON H. S. CHANG, ALAN BROWN, GORDON D. KOOP, BEN F. DAVIDSON, WILLIAM S. 2005 http://dx.doi.org/10.1111/j.1471-8286.2005.01056.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01056.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01056.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Notes volume 5, issue 4, page 762-766 ISSN 1471-8278 1471-8286 journal-article 2005 crwiley https://doi.org/10.1111/j.1471-8286.2005.01056.x 2024-07-09T04:11:06Z Abstract The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty‐one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Molecular Ecology Notes 5 4 762 766 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty‐one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers. |
format |
Article in Journal/Newspaper |
author |
NG, SIEMON H. S. CHANG, ALAN BROWN, GORDON D. KOOP, BEN F. DAVIDSON, WILLIAM S. |
spellingShingle |
NG, SIEMON H. S. CHANG, ALAN BROWN, GORDON D. KOOP, BEN F. DAVIDSON, WILLIAM S. Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
author_facet |
NG, SIEMON H. S. CHANG, ALAN BROWN, GORDON D. KOOP, BEN F. DAVIDSON, WILLIAM S. |
author_sort |
NG, SIEMON H. S. |
title |
Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
title_short |
Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
title_full |
Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
title_fullStr |
Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
title_full_unstemmed |
Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags |
title_sort |
type i microsatellite markers from atlantic salmon ( salmo salar) expressed sequence tags |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1471-8286.2005.01056.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01056.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01056.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Molecular Ecology Notes volume 5, issue 4, page 762-766 ISSN 1471-8278 1471-8286 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1471-8286.2005.01056.x |
container_title |
Molecular Ecology Notes |
container_volume |
5 |
container_issue |
4 |
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762 |
op_container_end_page |
766 |
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1810431978676158464 |