Type I microsatellite markers from Atlantic salmon ( Salmo salar) expressed sequence tags

Abstract The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST databa...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: NG, SIEMON H. S., CHANG, ALAN, BROWN, GORDON D., KOOP, BEN F., DAVIDSON, WILLIAM S.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2005
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1111/j.1471-8286.2005.01056.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01056.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01056.x
Description
Summary:Abstract The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty‐one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers.

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