Primers for sequence characterization and polymorphism detection in the Atlantic salmon ( Salmo salar) growth hormone 1 ( GH1) gene

• Published in: Molecular Ecology Notes
• Main Authors: RYYNÄNEN, HEIKKI J., PRIMMER, CRAIG R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2004
• Subjects: Indel’; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2004.00776.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2004.00776.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2004.00776.x

Abstract We report the identification of intraspecific sequence variation in the Atlantic salmon ( Salmo salar ) growth hormone 1 gene. Rapid and inexpensive assays for polymorphism detection were developed for 10 sites. Five of the assays detected single nucleotide polymorphisms (SNPs) using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analyses, and five were indel polymorphisms, detected using fragment length analyses. The average within population frequency of the most common allele varied from 0.52 to 0.90, and the average within population heterozygosity varied from 0.02 to 0.37 in seven European salmon populations.