Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides
Abstract We investigated the molecular mechanism underlying the ganglioside‐induced initiation of the assembly of wild and hereditary variant‐type amyloid β‐proteins, including Arctic‐, Dutch‐, and Flemish‐type amyloid β‐proteins. We monitored the assembly of amyloid β‐protein by thioflavin‐T assay,...
Published in: | Journal of Neurochemistry |
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Online Access: | http://dx.doi.org/10.1111/j.1471-4159.2005.03444.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.2005.03444.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2005.03444.x |
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crwiley:10.1111/j.1471-4159.2005.03444.x 2024-06-02T08:01:41+00:00 Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides Yamamoto, Naoki Matsuzaki, Katsumi Yanagisawa, Katsuhiko 2005 http://dx.doi.org/10.1111/j.1471-4159.2005.03444.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.2005.03444.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2005.03444.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Neurochemistry volume 95, issue 4, page 1167-1176 ISSN 0022-3042 1471-4159 journal-article 2005 crwiley https://doi.org/10.1111/j.1471-4159.2005.03444.x 2024-05-03T11:29:27Z Abstract We investigated the molecular mechanism underlying the ganglioside‐induced initiation of the assembly of wild and hereditary variant‐type amyloid β‐proteins, including Arctic‐, Dutch‐, and Flemish‐type amyloid β‐proteins. We monitored the assembly of amyloid β‐protein by thioflavin‐T assay, western blotting and electron microscopy. We also examined how externally added amyloid β‐protein assembles in a cell culture. The assembly of wild‐, Arctic‐, Dutch‐, and Flemish‐type amyloid β‐proteins were accelerated in the presence of GM1, GM1, GM3 and GD3 gangliosides. Notably, all of these amyloid β‐proteins accelerated the assembly of different type of amyloid β‐protein, following prior binding to a specific ganglioside. A specific‐ganglioside‐bound form of variant‐type amyloid β‐protein was recognized by the antibody (4396C) specific to the GM1‐ganglioside‐induced altered conformation of wild‐type amyloid β‐protein. Moreover, the assembly of these amyloid β‐proteins in the presence of a specific ganglioside was markedly suppressed by coincubation with 4396C. This study suggests that cross‐seeding can occur between wild and hereditary variant‐type amyloid β‐proteins despite differences in their amino acid sequences. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Journal of Neurochemistry 95 4 1167 1176 |
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description |
Abstract We investigated the molecular mechanism underlying the ganglioside‐induced initiation of the assembly of wild and hereditary variant‐type amyloid β‐proteins, including Arctic‐, Dutch‐, and Flemish‐type amyloid β‐proteins. We monitored the assembly of amyloid β‐protein by thioflavin‐T assay, western blotting and electron microscopy. We also examined how externally added amyloid β‐protein assembles in a cell culture. The assembly of wild‐, Arctic‐, Dutch‐, and Flemish‐type amyloid β‐proteins were accelerated in the presence of GM1, GM1, GM3 and GD3 gangliosides. Notably, all of these amyloid β‐proteins accelerated the assembly of different type of amyloid β‐protein, following prior binding to a specific ganglioside. A specific‐ganglioside‐bound form of variant‐type amyloid β‐protein was recognized by the antibody (4396C) specific to the GM1‐ganglioside‐induced altered conformation of wild‐type amyloid β‐protein. Moreover, the assembly of these amyloid β‐proteins in the presence of a specific ganglioside was markedly suppressed by coincubation with 4396C. This study suggests that cross‐seeding can occur between wild and hereditary variant‐type amyloid β‐proteins despite differences in their amino acid sequences. |
format |
Article in Journal/Newspaper |
author |
Yamamoto, Naoki Matsuzaki, Katsumi Yanagisawa, Katsuhiko |
spellingShingle |
Yamamoto, Naoki Matsuzaki, Katsumi Yanagisawa, Katsuhiko Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
author_facet |
Yamamoto, Naoki Matsuzaki, Katsumi Yanagisawa, Katsuhiko |
author_sort |
Yamamoto, Naoki |
title |
Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
title_short |
Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
title_full |
Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
title_fullStr |
Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
title_full_unstemmed |
Cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
title_sort |
cross‐seeding of wild‐type and hereditary variant‐type amyloid β‐proteins in the presence of gangliosides |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1111/j.1471-4159.2005.03444.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.2005.03444.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2005.03444.x |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
Journal of Neurochemistry volume 95, issue 4, page 1167-1176 ISSN 0022-3042 1471-4159 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1471-4159.2005.03444.x |
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Journal of Neurochemistry |
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95 |
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4 |
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1167 |
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1176 |
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1800746086546014208 |