The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants
Summary The role of acyl‐CoA‐dependent Δ6‐desaturation in the heterologous synthesis of omega‐3 long‐chain polyunsaturated fatty acids was systematically evaluated in transgenic yeast and Arabidopsis thaliana . The acyl‐CoA Δ6‐desaturase from the picoalga Ostreococcus tauri and orthologous activitie...
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crwiley:10.1111/j.1467-7652.2011.00653.x 2024-06-23T07:56:30+00:00 The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants Sayanova, Olga Ruiz‐Lopez, Noemi Haslam, Richard P. Napier, Johnathan A. 2011 http://dx.doi.org/10.1111/j.1467-7652.2011.00653.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1467-7652.2011.00653.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1467-7652.2011.00653.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Plant Biotechnology Journal volume 10, issue 2, page 195-206 ISSN 1467-7644 1467-7652 journal-article 2011 crwiley https://doi.org/10.1111/j.1467-7652.2011.00653.x 2024-06-06T04:21:10Z Summary The role of acyl‐CoA‐dependent Δ6‐desaturation in the heterologous synthesis of omega‐3 long‐chain polyunsaturated fatty acids was systematically evaluated in transgenic yeast and Arabidopsis thaliana . The acyl‐CoA Δ6‐desaturase from the picoalga Ostreococcus tauri and orthologous activities from mouse ( Mus musculus ) and salmon ( Salmo salar ) were shown to generate substantial levels of Δ6‐desaturated acyl‐CoAs, in contrast to the phospholipid‐dependent Δ6‐desaturases from higher plants that failed to modify this metabolic pool. Transgenic plants expressing the acyl‐CoA Δ6‐desaturases from either O. tauri or salmon, in conjunction with the two additional activities required for the synthesis of C20 polyunsaturated fatty acids, contained higher levels of eicosapentaenoic acid compared with plants expressing the borage phospholipid‐dependent Δ6‐desaturase. The use of acyl‐CoA‐dependent Δ6‐desaturases almost completely abolished the accumulation of unwanted biosynthetic intermediates such as γ‐linolenic acid in total seed lipids. Expression of acyl‐CoA Δ6‐desaturases resulted in increased distribution of long‐chain polyunsaturated fatty acids in the polar lipids of transgenic plants, reflecting the larger substrate pool available for acylation by enzymes of the Kennedy pathway. Expression of the O. tauri Δ6‐desaturase in transgenic Camelina sativa plants also resulted in the accumulation of high levels of Δ6‐desaturated fatty acids. This study provides evidence for the efficacy of using acyl‐CoA‐dependent Δ6‐desaturases in the efficient metabolic engineering of transgenic plants with high value traits such as the synthesis of omega‐3 LC‐PUFAs. Article in Journal/Newspaper Salmo salar Wiley Online Library Plant Biotechnology Journal 10 2 195 206 |
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Wiley Online Library |
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English |
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Summary The role of acyl‐CoA‐dependent Δ6‐desaturation in the heterologous synthesis of omega‐3 long‐chain polyunsaturated fatty acids was systematically evaluated in transgenic yeast and Arabidopsis thaliana . The acyl‐CoA Δ6‐desaturase from the picoalga Ostreococcus tauri and orthologous activities from mouse ( Mus musculus ) and salmon ( Salmo salar ) were shown to generate substantial levels of Δ6‐desaturated acyl‐CoAs, in contrast to the phospholipid‐dependent Δ6‐desaturases from higher plants that failed to modify this metabolic pool. Transgenic plants expressing the acyl‐CoA Δ6‐desaturases from either O. tauri or salmon, in conjunction with the two additional activities required for the synthesis of C20 polyunsaturated fatty acids, contained higher levels of eicosapentaenoic acid compared with plants expressing the borage phospholipid‐dependent Δ6‐desaturase. The use of acyl‐CoA‐dependent Δ6‐desaturases almost completely abolished the accumulation of unwanted biosynthetic intermediates such as γ‐linolenic acid in total seed lipids. Expression of acyl‐CoA Δ6‐desaturases resulted in increased distribution of long‐chain polyunsaturated fatty acids in the polar lipids of transgenic plants, reflecting the larger substrate pool available for acylation by enzymes of the Kennedy pathway. Expression of the O. tauri Δ6‐desaturase in transgenic Camelina sativa plants also resulted in the accumulation of high levels of Δ6‐desaturated fatty acids. This study provides evidence for the efficacy of using acyl‐CoA‐dependent Δ6‐desaturases in the efficient metabolic engineering of transgenic plants with high value traits such as the synthesis of omega‐3 LC‐PUFAs. |
format |
Article in Journal/Newspaper |
author |
Sayanova, Olga Ruiz‐Lopez, Noemi Haslam, Richard P. Napier, Johnathan A. |
spellingShingle |
Sayanova, Olga Ruiz‐Lopez, Noemi Haslam, Richard P. Napier, Johnathan A. The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
author_facet |
Sayanova, Olga Ruiz‐Lopez, Noemi Haslam, Richard P. Napier, Johnathan A. |
author_sort |
Sayanova, Olga |
title |
The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
title_short |
The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
title_full |
The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
title_fullStr |
The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
title_full_unstemmed |
The role of Δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
title_sort |
role of δ6‐desaturase acyl‐carrier specificity in the efficient synthesis of long‐chain polyunsaturated fatty acids in transgenic plants |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1467-7652.2011.00653.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1467-7652.2011.00653.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1467-7652.2011.00653.x |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Plant Biotechnology Journal volume 10, issue 2, page 195-206 ISSN 1467-7644 1467-7652 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1467-7652.2011.00653.x |
container_title |
Plant Biotechnology Journal |
container_volume |
10 |
container_issue |
2 |
container_start_page |
195 |
op_container_end_page |
206 |
_version_ |
1802649615727591424 |