High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic
Summary Osedax worms are whale‐fall specialists that infiltrate whale bones with their root tissues. These are filled with endosymbiotic bacteria hypothesized to provide their hosts with nutrition by extracting organic compounds from the whale bones. We investigated the diversity and distribution of...
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2010.02299.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2010.02299.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2010.02299.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2010.02299.x 2024-09-15T18:22:31+00:00 High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic Verna, Caroline Ramette, Alban Wiklund, Helena Dahlgren, Thomas G. Glover, Adrian G. Gaill, Françoise Dubilier, Nicole 2010 http://dx.doi.org/10.1111/j.1462-2920.2010.02299.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2010.02299.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2010.02299.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 12, issue 8, page 2355-2370 ISSN 1462-2912 1462-2920 journal-article 2010 crwiley https://doi.org/10.1111/j.1462-2920.2010.02299.x 2024-08-30T04:11:51Z Summary Osedax worms are whale‐fall specialists that infiltrate whale bones with their root tissues. These are filled with endosymbiotic bacteria hypothesized to provide their hosts with nutrition by extracting organic compounds from the whale bones. We investigated the diversity and distribution of symbiotic bacteria in Osedax mucofloris from shallow‐water whale‐falls in the North Atlantic using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH). We observed a higher diversity of endosymbionts than previously described from other Osedax species. Endosymbiont sequences fell into eight phylogenetically distinct clusters (with 91.4–98.9% similarity between clusters), and considerable microdiversity within clusters (99.5–99.7% similarity) was observed. Statistical tests revealed a highly significant effect of the host individual on endosymbiont diversity and distribution, with 68% of the variability between clusters and 40% of the variability within clusters explained by this effect. FISH analyses showed that most host individuals were dominated by endosymbionts from a single cluster, with endosymbionts from less abundant clusters generally confined to peripheral root tissues. The observed diversity and distribution patterns indicate that the endosymbionts are transmitted horizontally from the environment with repeated infection events occurring as the host root tissues grow into the whale bones. Article in Journal/Newspaper North Atlantic Wiley Online Library Environmental Microbiology 12 8 2355 2370 |
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English |
description |
Summary Osedax worms are whale‐fall specialists that infiltrate whale bones with their root tissues. These are filled with endosymbiotic bacteria hypothesized to provide their hosts with nutrition by extracting organic compounds from the whale bones. We investigated the diversity and distribution of symbiotic bacteria in Osedax mucofloris from shallow‐water whale‐falls in the North Atlantic using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH). We observed a higher diversity of endosymbionts than previously described from other Osedax species. Endosymbiont sequences fell into eight phylogenetically distinct clusters (with 91.4–98.9% similarity between clusters), and considerable microdiversity within clusters (99.5–99.7% similarity) was observed. Statistical tests revealed a highly significant effect of the host individual on endosymbiont diversity and distribution, with 68% of the variability between clusters and 40% of the variability within clusters explained by this effect. FISH analyses showed that most host individuals were dominated by endosymbionts from a single cluster, with endosymbionts from less abundant clusters generally confined to peripheral root tissues. The observed diversity and distribution patterns indicate that the endosymbionts are transmitted horizontally from the environment with repeated infection events occurring as the host root tissues grow into the whale bones. |
format |
Article in Journal/Newspaper |
author |
Verna, Caroline Ramette, Alban Wiklund, Helena Dahlgren, Thomas G. Glover, Adrian G. Gaill, Françoise Dubilier, Nicole |
spellingShingle |
Verna, Caroline Ramette, Alban Wiklund, Helena Dahlgren, Thomas G. Glover, Adrian G. Gaill, Françoise Dubilier, Nicole High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
author_facet |
Verna, Caroline Ramette, Alban Wiklund, Helena Dahlgren, Thomas G. Glover, Adrian G. Gaill, Françoise Dubilier, Nicole |
author_sort |
Verna, Caroline |
title |
High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
title_short |
High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
title_full |
High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
title_fullStr |
High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
title_full_unstemmed |
High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic |
title_sort |
high symbiont diversity in the bone‐eating worm osedax mucofloris from shallow whale‐falls in the north atlantic |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2010.02299.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2010.02299.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2010.02299.x/fullpdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Environmental Microbiology volume 12, issue 8, page 2355-2370 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2010.02299.x |
container_title |
Environmental Microbiology |
container_volume |
12 |
container_issue |
8 |
container_start_page |
2355 |
op_container_end_page |
2370 |
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1810462374573899776 |