Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice
Summary Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenti...
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2009.02126.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2009.02126.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2009.02126.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2009.02126.x 2024-06-02T08:02:34+00:00 Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice Eddie, Brian Juhl, Andrew Krembs, Christopher Baysinger, Charles Neuer, Susanne 2010 http://dx.doi.org/10.1111/j.1462-2920.2009.02126.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2009.02126.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2009.02126.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 12, issue 3, page 797-809 ISSN 1462-2912 1462-2920 journal-article 2010 crwiley https://doi.org/10.1111/j.1462-2920.2009.02126.x 2024-05-03T11:07:36Z Summary Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom‐specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice. Article in Journal/Newspaper Arctic Point Barrow Sea ice Wiley Online Library Arctic Environmental Microbiology 12 3 797 809 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the community in relation to vertical depth within the cores, as well as light availability (mainly as variable snow cover) and nutrient concentrations. We applied a combination of epifluorescence microscopy, denaturing gradient gel electrophoresis and clone libraries of a section of the 18S rRNA gene in order to compare the community structure of the major eukaryotic microbial phylotypes in the ice. We find that the community composition of the sea ice is more affected by the depth horizon in the ice than by light availability, although there are significant differences in the abundance of some groups between light regimes. Epifluorescence microscopy shows a shift from predominantly heterotrophic life styles in the upper ice to autotrophy prevailing in the bottom ice. This is supported by the statistical analysis of the similarity between the samples based on the denaturing gradient gel electrophoresis banding patterns, which shows a clear difference between upper and lower ice sections with respect to phylotypes and their proportional abundance. Clone libraries constructed using diatom‐specific primers confirm the high diversity of diatoms in the sea ice, and support the microscopic counts. Evidence of protistan grazing upon diatoms was also found in lower sections of the core, with implications for carbon and nutrient recycling in the ice. |
format |
Article in Journal/Newspaper |
author |
Eddie, Brian Juhl, Andrew Krembs, Christopher Baysinger, Charles Neuer, Susanne |
spellingShingle |
Eddie, Brian Juhl, Andrew Krembs, Christopher Baysinger, Charles Neuer, Susanne Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
author_facet |
Eddie, Brian Juhl, Andrew Krembs, Christopher Baysinger, Charles Neuer, Susanne |
author_sort |
Eddie, Brian |
title |
Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
title_short |
Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
title_full |
Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
title_fullStr |
Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
title_full_unstemmed |
Effect of environmental variables on eukaryotic microbial community structure of land‐fast Arctic sea ice |
title_sort |
effect of environmental variables on eukaryotic microbial community structure of land‐fast arctic sea ice |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2009.02126.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2009.02126.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2009.02126.x/fullpdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Point Barrow Sea ice |
genre_facet |
Arctic Point Barrow Sea ice |
op_source |
Environmental Microbiology volume 12, issue 3, page 797-809 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2009.02126.x |
container_title |
Environmental Microbiology |
container_volume |
12 |
container_issue |
3 |
container_start_page |
797 |
op_container_end_page |
809 |
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1800747050869981184 |