Macromolecular synthesis by yeasts under frozen conditions
Summary Although viable fungi have been recovered from a wide variety of icy environments, their metabolic capabilities under frozen conditions are still largely unknown. We investigated basidiomycetous yeasts isolated from an Antarctic ice core and showed that after freezing at a relatively slow ra...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2009
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2008.01829.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01829.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01829.x/fullpdf |
id |
crwiley:10.1111/j.1462-2920.2008.01829.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1462-2920.2008.01829.x 2024-09-15T17:42:04+00:00 Macromolecular synthesis by yeasts under frozen conditions Amato, Pierre Doyle, Shawn Christner, Brent C. 2009 http://dx.doi.org/10.1111/j.1462-2920.2008.01829.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01829.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01829.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 11, issue 3, page 589-596 ISSN 1462-2912 1462-2920 journal-article 2009 crwiley https://doi.org/10.1111/j.1462-2920.2008.01829.x 2024-08-09T04:22:32Z Summary Although viable fungi have been recovered from a wide variety of icy environments, their metabolic capabilities under frozen conditions are still largely unknown. We investigated basidiomycetous yeasts isolated from an Antarctic ice core and showed that after freezing at a relatively slow rate (0.8°C min −1 ), the cells are excluded into veins of liquid at the triple junctions of ice crystals. These strains were capable of reproductive growth at −5°C under liquid conditions. Under frozen conditions, metabolic activity was assessed by measuring rates of [ 3 H]leucine incorporation into the acid‐insoluble macromolecular fraction, which decreased exponentially at temperatures between 15°C and −15°C and was inhibited by the protein synthesis inhibitor cycloheximide. Experiments at −5°C under frozen and liquid conditions revealed 2–3 orders of magnitude lower rates of endogenous metabolism in ice, likely due to the high salinity in the liquid fraction of the ice (equivalent of ≈ 1.4 mol l −1 of NaCl at −5°C). The mesophile Saccharomyces cerevisae also incorporated [ 3 H]leucine at −5°C and −15°C, indicating that this activity is not exclusive to cold‐adapted microorganisms. The ability of yeast cells to incorporate amino acid substrates into macromolecules and remain metabolically active under these conditions has implications for understanding the survival of Eukarya in icy environments. Article in Journal/Newspaper Antarc* Antarctic ice core Wiley Online Library Environmental Microbiology 11 3 589 596 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Summary Although viable fungi have been recovered from a wide variety of icy environments, their metabolic capabilities under frozen conditions are still largely unknown. We investigated basidiomycetous yeasts isolated from an Antarctic ice core and showed that after freezing at a relatively slow rate (0.8°C min −1 ), the cells are excluded into veins of liquid at the triple junctions of ice crystals. These strains were capable of reproductive growth at −5°C under liquid conditions. Under frozen conditions, metabolic activity was assessed by measuring rates of [ 3 H]leucine incorporation into the acid‐insoluble macromolecular fraction, which decreased exponentially at temperatures between 15°C and −15°C and was inhibited by the protein synthesis inhibitor cycloheximide. Experiments at −5°C under frozen and liquid conditions revealed 2–3 orders of magnitude lower rates of endogenous metabolism in ice, likely due to the high salinity in the liquid fraction of the ice (equivalent of ≈ 1.4 mol l −1 of NaCl at −5°C). The mesophile Saccharomyces cerevisae also incorporated [ 3 H]leucine at −5°C and −15°C, indicating that this activity is not exclusive to cold‐adapted microorganisms. The ability of yeast cells to incorporate amino acid substrates into macromolecules and remain metabolically active under these conditions has implications for understanding the survival of Eukarya in icy environments. |
format |
Article in Journal/Newspaper |
author |
Amato, Pierre Doyle, Shawn Christner, Brent C. |
spellingShingle |
Amato, Pierre Doyle, Shawn Christner, Brent C. Macromolecular synthesis by yeasts under frozen conditions |
author_facet |
Amato, Pierre Doyle, Shawn Christner, Brent C. |
author_sort |
Amato, Pierre |
title |
Macromolecular synthesis by yeasts under frozen conditions |
title_short |
Macromolecular synthesis by yeasts under frozen conditions |
title_full |
Macromolecular synthesis by yeasts under frozen conditions |
title_fullStr |
Macromolecular synthesis by yeasts under frozen conditions |
title_full_unstemmed |
Macromolecular synthesis by yeasts under frozen conditions |
title_sort |
macromolecular synthesis by yeasts under frozen conditions |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2008.01829.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01829.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01829.x/fullpdf |
genre |
Antarc* Antarctic ice core |
genre_facet |
Antarc* Antarctic ice core |
op_source |
Environmental Microbiology volume 11, issue 3, page 589-596 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01829.x |
container_title |
Environmental Microbiology |
container_volume |
11 |
container_issue |
3 |
container_start_page |
589 |
op_container_end_page |
596 |
_version_ |
1810488440356077568 |