Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils
Summary Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest ph...
Published in: | Environmental Microbiology |
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crwiley:10.1111/j.1462-2920.2008.01809.x 2024-06-23T07:47:41+00:00 Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils Babalola, Olubukola O. Kirby, Bronwyn M. Le Roes‐Hill, Marilize Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. 2009 http://dx.doi.org/10.1111/j.1462-2920.2008.01809.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01809.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01809.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 11, issue 3, page 566-576 ISSN 1462-2912 1462-2920 journal-article 2009 crwiley https://doi.org/10.1111/j.1462-2920.2008.01809.x 2024-06-04T06:43:41Z Summary Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. Previous metagenomic studies have shown that the majority of Antarctic actinobacterial populations are classified as ‘uncultured’. In this study, we assessed the diversity of actinobacteria in Antarctic cold desert soils by complementing traditional culture‐based techniques with a metagenomic study. Phylogenetic analysis of clones generated with actinobacterium‐ and streptomycete‐specific PCR primers revealed that the majority of the phylotypes were most closely related to uncultured Pseudonocardia and Nocardioides species. Phylotypes most closely related to a number of rarer actinobacteria genera, including Geodermatophilus , Modestobacter and Sporichthya , were also identified. While complementary culture‐dependent studies isolated a number of Nocardia and Pseudonocardia species, the majority of the cultured isolates (> 80%) were S treptomyces species – although phylotypes affiliated to the genus Streptomyces were detected at a low frequency in the metagenomic study. This study confirms that Antarctic Dry Valley desert soil harbours highly diverse actinobacterial communities and suggests that many of the phylotypes identified may represent novel, uncultured species. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Wiley Online Library Antarctic McMurdo Dry Valleys Environmental Microbiology 11 3 566 576 |
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Wiley Online Library |
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language |
English |
description |
Summary Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. Previous metagenomic studies have shown that the majority of Antarctic actinobacterial populations are classified as ‘uncultured’. In this study, we assessed the diversity of actinobacteria in Antarctic cold desert soils by complementing traditional culture‐based techniques with a metagenomic study. Phylogenetic analysis of clones generated with actinobacterium‐ and streptomycete‐specific PCR primers revealed that the majority of the phylotypes were most closely related to uncultured Pseudonocardia and Nocardioides species. Phylotypes most closely related to a number of rarer actinobacteria genera, including Geodermatophilus , Modestobacter and Sporichthya , were also identified. While complementary culture‐dependent studies isolated a number of Nocardia and Pseudonocardia species, the majority of the cultured isolates (> 80%) were S treptomyces species – although phylotypes affiliated to the genus Streptomyces were detected at a low frequency in the metagenomic study. This study confirms that Antarctic Dry Valley desert soil harbours highly diverse actinobacterial communities and suggests that many of the phylotypes identified may represent novel, uncultured species. |
format |
Article in Journal/Newspaper |
author |
Babalola, Olubukola O. Kirby, Bronwyn M. Le Roes‐Hill, Marilize Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. |
spellingShingle |
Babalola, Olubukola O. Kirby, Bronwyn M. Le Roes‐Hill, Marilize Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
author_facet |
Babalola, Olubukola O. Kirby, Bronwyn M. Le Roes‐Hill, Marilize Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. |
author_sort |
Babalola, Olubukola O. |
title |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_short |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_full |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_fullStr |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_full_unstemmed |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_sort |
phylogenetic analysis of actinobacterial populations associated with antarctic dry valley mineral soils |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2008.01809.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01809.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01809.x/fullpdf |
geographic |
Antarctic McMurdo Dry Valleys |
geographic_facet |
Antarctic McMurdo Dry Valleys |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_source |
Environmental Microbiology volume 11, issue 3, page 566-576 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01809.x |
container_title |
Environmental Microbiology |
container_volume |
11 |
container_issue |
3 |
container_start_page |
566 |
op_container_end_page |
576 |
_version_ |
1802651832982437888 |