Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes
Summary We collected surface samples in Franklin Bay (Western Arctic) from ice‐covered to ice‐free conditions, to determine seasonal changes in the identity and in situ activity of the prokaryotic assemblages. Catalysed reported fluorescence in situ hybridization was used to quantify the abundance o...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2008.01674.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01674.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01674.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2008.01674.x 2024-06-02T08:01:40+00:00 Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes Alonso‐Sáez, Laura Sánchez, Olga Gasol, Josep M. Balagué, Vanessa Pedrós‐Alio, Carlos 2008 http://dx.doi.org/10.1111/j.1462-2920.2008.01674.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01674.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01674.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 10, issue 9, page 2444-2454 ISSN 1462-2912 1462-2920 journal-article 2008 crwiley https://doi.org/10.1111/j.1462-2920.2008.01674.x 2024-05-03T11:15:48Z Summary We collected surface samples in Franklin Bay (Western Arctic) from ice‐covered to ice‐free conditions, to determine seasonal changes in the identity and in situ activity of the prokaryotic assemblages. Catalysed reported fluorescence in situ hybridization was used to quantify the abundance of different groups, and combined with microautoradiography to determine the fraction of active cells taking up three substrates: glucose, amino acids and ATP. In surface waters, Archaea accounted for 16% of the total cell count in winter, but decreased to almost undetectable levels in summer, when Bacteria made up 97% of the total cell count. Alphaproteobacteria were the most abundant group followed by Bacteroidetes (average of 34% and 14% of total cell counts respectively). Some bacterial groups appearing in low abundances (< 10% of total cell counts), such as Betaproteobacteria , Roseobacter and Gammaproteobacteria , showed a high percentage of active cells. By contrast, more abundant groups, such as SAR11 or Bacteroidetes , had a lower percentage of active cells in the uptake of the substrates tested. Archaea showed low heterotrophic activity throughout the year. In comparison with temperate oceans, the percentage of active Bacteria in the uptake of the substrates was relatively high, even during the winter season. Article in Journal/Newspaper Arctic Franklin Bay Wiley Online Library Arctic Environmental Microbiology 10 9 2444 2454 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Summary We collected surface samples in Franklin Bay (Western Arctic) from ice‐covered to ice‐free conditions, to determine seasonal changes in the identity and in situ activity of the prokaryotic assemblages. Catalysed reported fluorescence in situ hybridization was used to quantify the abundance of different groups, and combined with microautoradiography to determine the fraction of active cells taking up three substrates: glucose, amino acids and ATP. In surface waters, Archaea accounted for 16% of the total cell count in winter, but decreased to almost undetectable levels in summer, when Bacteria made up 97% of the total cell count. Alphaproteobacteria were the most abundant group followed by Bacteroidetes (average of 34% and 14% of total cell counts respectively). Some bacterial groups appearing in low abundances (< 10% of total cell counts), such as Betaproteobacteria , Roseobacter and Gammaproteobacteria , showed a high percentage of active cells. By contrast, more abundant groups, such as SAR11 or Bacteroidetes , had a lower percentage of active cells in the uptake of the substrates tested. Archaea showed low heterotrophic activity throughout the year. In comparison with temperate oceans, the percentage of active Bacteria in the uptake of the substrates was relatively high, even during the winter season. |
format |
Article in Journal/Newspaper |
author |
Alonso‐Sáez, Laura Sánchez, Olga Gasol, Josep M. Balagué, Vanessa Pedrós‐Alio, Carlos |
spellingShingle |
Alonso‐Sáez, Laura Sánchez, Olga Gasol, Josep M. Balagué, Vanessa Pedrós‐Alio, Carlos Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
author_facet |
Alonso‐Sáez, Laura Sánchez, Olga Gasol, Josep M. Balagué, Vanessa Pedrós‐Alio, Carlos |
author_sort |
Alonso‐Sáez, Laura |
title |
Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
title_short |
Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
title_full |
Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
title_fullStr |
Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
title_full_unstemmed |
Winter‐to‐summer changes in the composition and single‐cell activity of near‐surface Arctic prokaryotes |
title_sort |
winter‐to‐summer changes in the composition and single‐cell activity of near‐surface arctic prokaryotes |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2008.01674.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01674.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01674.x/fullpdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Franklin Bay |
genre_facet |
Arctic Franklin Bay |
op_source |
Environmental Microbiology volume 10, issue 9, page 2444-2454 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01674.x |
container_title |
Environmental Microbiology |
container_volume |
10 |
container_issue |
9 |
container_start_page |
2444 |
op_container_end_page |
2454 |
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1800746076388458496 |