Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy
Summary Bacteriohopanoids are widespread lipid biomarkers in the sedimentary record. Many aerobic and anaerobic bacteria are potential sources of these lipids which sometimes complicates the use of these biomarkers as proxies for ecological and environmental changes. Therefore, we applied preserved...
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crwiley:10.1111/j.1462-2920.2008.01601.x 2024-09-15T17:44:38+00:00 Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy Coolen, Marco J. L. Talbot, Helen M. Abbas, Ben A. Ward, Christopher Schouten, Stefan Volkman, John K. Damsté, Jaap S. Sinninghe 2008 http://dx.doi.org/10.1111/j.1462-2920.2008.01601.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01601.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01601.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 10, issue 7, page 1783-1803 ISSN 1462-2912 1462-2920 journal-article 2008 crwiley https://doi.org/10.1111/j.1462-2920.2008.01601.x 2024-07-30T04:18:55Z Summary Bacteriohopanoids are widespread lipid biomarkers in the sedimentary record. Many aerobic and anaerobic bacteria are potential sources of these lipids which sometimes complicates the use of these biomarkers as proxies for ecological and environmental changes. Therefore, we applied preserved 16S ribosomal RNA genes to identify likely Holocene biological sources of bacteriohopanepolyols (BHPs) in the sulfidic sediments of the permanently stratified postglacial Ace Lake, Antarctica. A suite of intact BHPs were identified, which revealed a variety of structural forms whose composition differed through the sediment core reflecting changes in bacterial populations induced by large changes in lake salinity. Stable isotopic compositions of the hopanols formed from periodic acid‐cleaved BHPs, showed that some were substantially depleted in 13 C, indicative of their methanotrophic origin. Using sensitive molecular tools, we found that Type I and II methanotrophic bacteria (respectively Methylomonas and Methylocystis ) were unique to the oldest lacustrine sediments (> 9400 years BP), but quantification of fossil DNA revealed that the Type I methanotrophs, including methanotrophs related to methanotrophic gill symbionts of deep‐sea cold‐seep mussels, were the main precursors of the 35‐amino BHPs (i.e. aminopentol, ‐tetrol and ‐triols). After isolation of the lake ∼3000 years ago, one Type I methanotroph of the ‘methanotrophic gill symbionts cluster’ remained the most obvious source of aminotetrol and ‐triol. We, furthermore, identified a Synechococcus phylotype related to pelagic freshwater strains in the oldest lacustrine sediments as a putative source of 2‐methylbacteriohopanetetrol (2‐Me BHT). This combined application of advanced geochemical and paleogenomical tools further refined our knowledge about Holocene biogeochemical processes in Ace Lake. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Environmental Microbiology 10 7 1783 1803 |
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Wiley Online Library |
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crwiley |
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English |
description |
Summary Bacteriohopanoids are widespread lipid biomarkers in the sedimentary record. Many aerobic and anaerobic bacteria are potential sources of these lipids which sometimes complicates the use of these biomarkers as proxies for ecological and environmental changes. Therefore, we applied preserved 16S ribosomal RNA genes to identify likely Holocene biological sources of bacteriohopanepolyols (BHPs) in the sulfidic sediments of the permanently stratified postglacial Ace Lake, Antarctica. A suite of intact BHPs were identified, which revealed a variety of structural forms whose composition differed through the sediment core reflecting changes in bacterial populations induced by large changes in lake salinity. Stable isotopic compositions of the hopanols formed from periodic acid‐cleaved BHPs, showed that some were substantially depleted in 13 C, indicative of their methanotrophic origin. Using sensitive molecular tools, we found that Type I and II methanotrophic bacteria (respectively Methylomonas and Methylocystis ) were unique to the oldest lacustrine sediments (> 9400 years BP), but quantification of fossil DNA revealed that the Type I methanotrophs, including methanotrophs related to methanotrophic gill symbionts of deep‐sea cold‐seep mussels, were the main precursors of the 35‐amino BHPs (i.e. aminopentol, ‐tetrol and ‐triols). After isolation of the lake ∼3000 years ago, one Type I methanotroph of the ‘methanotrophic gill symbionts cluster’ remained the most obvious source of aminotetrol and ‐triol. We, furthermore, identified a Synechococcus phylotype related to pelagic freshwater strains in the oldest lacustrine sediments as a putative source of 2‐methylbacteriohopanetetrol (2‐Me BHT). This combined application of advanced geochemical and paleogenomical tools further refined our knowledge about Holocene biogeochemical processes in Ace Lake. |
format |
Article in Journal/Newspaper |
author |
Coolen, Marco J. L. Talbot, Helen M. Abbas, Ben A. Ward, Christopher Schouten, Stefan Volkman, John K. Damsté, Jaap S. Sinninghe |
spellingShingle |
Coolen, Marco J. L. Talbot, Helen M. Abbas, Ben A. Ward, Christopher Schouten, Stefan Volkman, John K. Damsté, Jaap S. Sinninghe Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
author_facet |
Coolen, Marco J. L. Talbot, Helen M. Abbas, Ben A. Ward, Christopher Schouten, Stefan Volkman, John K. Damsté, Jaap S. Sinninghe |
author_sort |
Coolen, Marco J. L. |
title |
Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
title_short |
Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
title_full |
Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
title_fullStr |
Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
title_full_unstemmed |
Sources for sedimentary bacteriohopanepolyols as revealed by 16S rDNA stratigraphy |
title_sort |
sources for sedimentary bacteriohopanepolyols as revealed by 16s rdna stratigraphy |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2008.01601.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2008.01601.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2008.01601.x/fullpdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Environmental Microbiology volume 10, issue 7, page 1783-1803 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01601.x |
container_title |
Environmental Microbiology |
container_volume |
10 |
container_issue |
7 |
container_start_page |
1783 |
op_container_end_page |
1803 |
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1810492276858683392 |