Prokaryotic diversity of arctic ice shelf microbial mats
Summary The prokaryotic diversity and respiratory activity of microbial mat communities on the Markham Ice Shelf and Ward Hunt Ice Shelf in the Canadian high Arctic were analysed. All heterotrophic isolates and > 95% of bacterial 16S rRNA gene clone library sequences from both ice shelves grouped...
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2007.01516.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01516.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01516.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2007.01516.x 2024-06-23T07:49:43+00:00 Prokaryotic diversity of arctic ice shelf microbial mats Bottos, Eric M. Vincent, Warwick F. Greer, Charles W. Whyte, Lyle G. 2008 http://dx.doi.org/10.1111/j.1462-2920.2007.01516.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01516.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01516.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 10, issue 4, page 950-966 ISSN 1462-2912 1462-2920 journal-article 2008 crwiley https://doi.org/10.1111/j.1462-2920.2007.01516.x 2024-06-04T06:43:10Z Summary The prokaryotic diversity and respiratory activity of microbial mat communities on the Markham Ice Shelf and Ward Hunt Ice Shelf in the Canadian high Arctic were analysed. All heterotrophic isolates and > 95% of bacterial 16S rRNA gene clone library sequences from both ice shelves grouped within the phyla Bacteroidetes , Proteobacteria and Actinobacteria . Clone library analyses showed that the bacterial communities were diverse and varied significantly between the two ice shelves, with the Markham library having a higher estimated diversity (Chao1 = 243; 105 operational taxonomic units observed in 189 clones) than the Ward Hunt library (Chao1 = 106; 52 operational taxonomic units observed in 128 clones). Archaeal 16S rRNA gene clone libraries from both ice shelves were dominated by a single Euryarchaeota sequence, which appears to represent a novel phylotype. Analyses of community activity by radiorespiration assays detected metabolism in mat samples from both ice shelves at temperatures as low as −10°C. These findings provide the first insight into the prokaryotic biodiversity of Arctic ice shelf communities and underscore the importance of these cryo‐ecosystems as a rich source of microbiota that are adapted to extreme cold. Article in Journal/Newspaper Arctic Ice Shelf Ice Shelves Markham Ice Shelf Ward Hunt Ice Shelf Wiley Online Library Arctic Markham ENVELOPE(-57.358,-57.358,-64.296,-64.296) Ward Hunt Ice Shelf ENVELOPE(-73.499,-73.499,83.122,83.122) Environmental Microbiology 10 4 950 966 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary The prokaryotic diversity and respiratory activity of microbial mat communities on the Markham Ice Shelf and Ward Hunt Ice Shelf in the Canadian high Arctic were analysed. All heterotrophic isolates and > 95% of bacterial 16S rRNA gene clone library sequences from both ice shelves grouped within the phyla Bacteroidetes , Proteobacteria and Actinobacteria . Clone library analyses showed that the bacterial communities were diverse and varied significantly between the two ice shelves, with the Markham library having a higher estimated diversity (Chao1 = 243; 105 operational taxonomic units observed in 189 clones) than the Ward Hunt library (Chao1 = 106; 52 operational taxonomic units observed in 128 clones). Archaeal 16S rRNA gene clone libraries from both ice shelves were dominated by a single Euryarchaeota sequence, which appears to represent a novel phylotype. Analyses of community activity by radiorespiration assays detected metabolism in mat samples from both ice shelves at temperatures as low as −10°C. These findings provide the first insight into the prokaryotic biodiversity of Arctic ice shelf communities and underscore the importance of these cryo‐ecosystems as a rich source of microbiota that are adapted to extreme cold. |
format |
Article in Journal/Newspaper |
author |
Bottos, Eric M. Vincent, Warwick F. Greer, Charles W. Whyte, Lyle G. |
spellingShingle |
Bottos, Eric M. Vincent, Warwick F. Greer, Charles W. Whyte, Lyle G. Prokaryotic diversity of arctic ice shelf microbial mats |
author_facet |
Bottos, Eric M. Vincent, Warwick F. Greer, Charles W. Whyte, Lyle G. |
author_sort |
Bottos, Eric M. |
title |
Prokaryotic diversity of arctic ice shelf microbial mats |
title_short |
Prokaryotic diversity of arctic ice shelf microbial mats |
title_full |
Prokaryotic diversity of arctic ice shelf microbial mats |
title_fullStr |
Prokaryotic diversity of arctic ice shelf microbial mats |
title_full_unstemmed |
Prokaryotic diversity of arctic ice shelf microbial mats |
title_sort |
prokaryotic diversity of arctic ice shelf microbial mats |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2007.01516.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01516.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01516.x/fullpdf |
long_lat |
ENVELOPE(-57.358,-57.358,-64.296,-64.296) ENVELOPE(-73.499,-73.499,83.122,83.122) |
geographic |
Arctic Markham Ward Hunt Ice Shelf |
geographic_facet |
Arctic Markham Ward Hunt Ice Shelf |
genre |
Arctic Ice Shelf Ice Shelves Markham Ice Shelf Ward Hunt Ice Shelf |
genre_facet |
Arctic Ice Shelf Ice Shelves Markham Ice Shelf Ward Hunt Ice Shelf |
op_source |
Environmental Microbiology volume 10, issue 4, page 950-966 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2007.01516.x |
container_title |
Environmental Microbiology |
container_volume |
10 |
container_issue |
4 |
container_start_page |
950 |
op_container_end_page |
966 |
_version_ |
1802640398901837824 |