Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity
Summary Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied to substantiate this claim. In a combined approach using the...
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2007.01266.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01266.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01266.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2007.01266.x 2024-09-09T19:32:15+00:00 Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity Schmid, Markus C. Risgaard‐Petersen, Nils Van De Vossenberg, Jack Kuypers, Marcel M. M. Lavik, Gaute Petersen, Jan Hulth, Stefan Thamdrup, Bo Canfield, Don Dalsgaard, Tage Rysgaard, Søren Sejr, Mikael K. Strous, Marc Op den Camp, Huub J. M. Jetten, Mike S. M. 2007 http://dx.doi.org/10.1111/j.1462-2920.2007.01266.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01266.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01266.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 9, issue 6, page 1476-1484 ISSN 1462-2912 1462-2920 journal-article 2007 crwiley https://doi.org/10.1111/j.1462-2920.2007.01266.x 2024-08-20T04:17:01Z Summary Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied to substantiate this claim. In a combined approach using the molecular methods, polymerase chain reaction (PCR), qualitative and quantitative fluorescence in situ hybridization (FISH), as well as 15 N stable isotope activity measurements, it was shown that anammox bacteria were present and active in all samples investigated. The anammox activity measured in the sediment samples ranged from 0.08 fmol cell −1 day −1 N 2 in the Golfo Dulce (Pacific Ocean, Costa Rica) sediment to 0.98 fmol cell −1 day −1 N 2 in the Gullmarsfjorden (North Sea, Sweden) sediment. The percentage of anammox cell of the total population (stained with DAPI) as assessed by quantitative FISH was highest in the Barents Sea (9% ± 4%) and in most of the samples well over 2%. Fluorescence in situ hybridization and phylogenetic analysis of the PCR products derived from the marine samples indicated the exclusive presence of members of the Candidatus ‘Scalindua’ genus. This study showed the ubiquitous presence of anammox bacteria in anoxic marine ecosystems, supporting previous observations on the importance of anammox for N cycling in marine environments. Article in Journal/Newspaper Barents Sea Wiley Online Library Barents Sea Pacific Environmental Microbiology 9 6 1476 1484 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied to substantiate this claim. In a combined approach using the molecular methods, polymerase chain reaction (PCR), qualitative and quantitative fluorescence in situ hybridization (FISH), as well as 15 N stable isotope activity measurements, it was shown that anammox bacteria were present and active in all samples investigated. The anammox activity measured in the sediment samples ranged from 0.08 fmol cell −1 day −1 N 2 in the Golfo Dulce (Pacific Ocean, Costa Rica) sediment to 0.98 fmol cell −1 day −1 N 2 in the Gullmarsfjorden (North Sea, Sweden) sediment. The percentage of anammox cell of the total population (stained with DAPI) as assessed by quantitative FISH was highest in the Barents Sea (9% ± 4%) and in most of the samples well over 2%. Fluorescence in situ hybridization and phylogenetic analysis of the PCR products derived from the marine samples indicated the exclusive presence of members of the Candidatus ‘Scalindua’ genus. This study showed the ubiquitous presence of anammox bacteria in anoxic marine ecosystems, supporting previous observations on the importance of anammox for N cycling in marine environments. |
format |
Article in Journal/Newspaper |
author |
Schmid, Markus C. Risgaard‐Petersen, Nils Van De Vossenberg, Jack Kuypers, Marcel M. M. Lavik, Gaute Petersen, Jan Hulth, Stefan Thamdrup, Bo Canfield, Don Dalsgaard, Tage Rysgaard, Søren Sejr, Mikael K. Strous, Marc Op den Camp, Huub J. M. Jetten, Mike S. M. |
spellingShingle |
Schmid, Markus C. Risgaard‐Petersen, Nils Van De Vossenberg, Jack Kuypers, Marcel M. M. Lavik, Gaute Petersen, Jan Hulth, Stefan Thamdrup, Bo Canfield, Don Dalsgaard, Tage Rysgaard, Søren Sejr, Mikael K. Strous, Marc Op den Camp, Huub J. M. Jetten, Mike S. M. Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
author_facet |
Schmid, Markus C. Risgaard‐Petersen, Nils Van De Vossenberg, Jack Kuypers, Marcel M. M. Lavik, Gaute Petersen, Jan Hulth, Stefan Thamdrup, Bo Canfield, Don Dalsgaard, Tage Rysgaard, Søren Sejr, Mikael K. Strous, Marc Op den Camp, Huub J. M. Jetten, Mike S. M. |
author_sort |
Schmid, Markus C. |
title |
Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_short |
Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_full |
Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_fullStr |
Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_full_unstemmed |
Anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_sort |
anaerobic ammonium‐oxidizing bacteria in marine environments: widespread occurrence but low diversity |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2007.01266.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2007.01266.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2007.01266.x/fullpdf |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Environmental Microbiology volume 9, issue 6, page 1476-1484 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2007.01266.x |
container_title |
Environmental Microbiology |
container_volume |
9 |
container_issue |
6 |
container_start_page |
1476 |
op_container_end_page |
1484 |
_version_ |
1809901080878776320 |