Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox
Summary The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m...
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2006.01087.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01087.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01087.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2006.01087.x 2024-09-15T17:45:06+00:00 Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox Lavire, Céline Normand, Philippe Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean‐Louis Fournier, Pascale Hänni, Catherine Petit, Jean‐Robert 2006 http://dx.doi.org/10.1111/j.1462-2920.2006.01087.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01087.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01087.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 8, issue 12, page 2106-2114 ISSN 1462-2912 1462-2920 journal-article 2006 crwiley https://doi.org/10.1111/j.1462-2920.2006.01087.x 2024-08-13T04:13:32Z Summary The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular‐phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene ( rrs ) targeted primers. As a result, rrs ‐targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus (< 1%). In addition, RubisCO ( cbbL or rbcL ) and NiFe‐Hydrogenase (hoxV or hupL ) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus . All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells. Article in Journal/Newspaper Antarc* Antarctica ice core Wiley Online Library Environmental Microbiology 8 12 2106 2114 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Summary The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular‐phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene ( rrs ) targeted primers. As a result, rrs ‐targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus (< 1%). In addition, RubisCO ( cbbL or rbcL ) and NiFe‐Hydrogenase (hoxV or hupL ) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus . All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells. |
format |
Article in Journal/Newspaper |
author |
Lavire, Céline Normand, Philippe Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean‐Louis Fournier, Pascale Hänni, Catherine Petit, Jean‐Robert |
spellingShingle |
Lavire, Céline Normand, Philippe Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean‐Louis Fournier, Pascale Hänni, Catherine Petit, Jean‐Robert Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
author_facet |
Lavire, Céline Normand, Philippe Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean‐Louis Fournier, Pascale Hänni, Catherine Petit, Jean‐Robert |
author_sort |
Lavire, Céline |
title |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_short |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_full |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_fullStr |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_full_unstemmed |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_sort |
presence of hydrogenophilus thermoluteolus dna in accretion ice in the subglacial lake vostok, antarctica, assessed using rrs, cbb and hox |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2006.01087.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01087.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01087.x/fullpdf |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_source |
Environmental Microbiology volume 8, issue 12, page 2106-2114 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01087.x |
container_title |
Environmental Microbiology |
container_volume |
8 |
container_issue |
12 |
container_start_page |
2106 |
op_container_end_page |
2114 |
_version_ |
1810492817029464064 |