Site‐specific variation in Antarctic marine biofilms established on artificial surfaces
Summary The community structure and composition of marine microbial biofilms established on glass surfaces was investigated across three differentially contaminated Antarctic sites within McMurdo Sound. Diverse microbial communities were revealed at all sites using fluorescence in situ hybridization...
Published in: | Environmental Microbiology |
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Online Access: | http://dx.doi.org/10.1111/j.1462-2920.2006.01007.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01007.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01007.x/fullpdf |
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crwiley:10.1111/j.1462-2920.2006.01007.x 2024-06-23T07:47:51+00:00 Site‐specific variation in Antarctic marine biofilms established on artificial surfaces Webster, Nicole S. Negri, Andrew P. 2006 http://dx.doi.org/10.1111/j.1462-2920.2006.01007.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01007.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01007.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology volume 8, issue 7, page 1177-1190 ISSN 1462-2912 1462-2920 journal-article 2006 crwiley https://doi.org/10.1111/j.1462-2920.2006.01007.x 2024-06-13T04:24:28Z Summary The community structure and composition of marine microbial biofilms established on glass surfaces was investigated across three differentially contaminated Antarctic sites within McMurdo Sound. Diverse microbial communities were revealed at all sites using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques. Sequencing of excised DGGE bands demonstrated close affiliation with known psychrophiles or undescribed bacteria also recovered from the Antarctic environment. The majority of bacterial sequences were affiliated to the Gammaproteobacteria , Cytophaga/Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia and Planctomycetales . Principal components analysis of quantitative FISH data revealed distinct differences in community composition between sites. Each of the sites were dominated by different bacterial groups: Alphaproteobacteria , Gammaproteobacteria and CFB at the least impacted site, Cape Armitage; green sulfur and sulfate reducing bacteria near the semi‐impacted Scott Base and Planctomycetales and sulfate reducing bacteria near the highly impacted McMurdo Station. The highest abundance of archaea was detected near Scott Base (2.5% of total bacteria). Multivariate analyses (non‐metric multidimensional scaling and analysis of similarities) of DGGE patterns revealed greater variability in community composition between sites than within sites. This is the first investigation of Antarctic biofilm structure and FISH results suggest that anthropogenic impacts may influence the complex composition of microbial communities. Article in Journal/Newspaper Antarc* Antarctic McMurdo Sound Wiley Online Library Antarctic Armitage ENVELOPE(166.667,166.667,-77.850,-77.850) Cape Armitage ENVELOPE(163.250,163.250,-78.150,-78.150) McMurdo Sound McMurdo Station ENVELOPE(166.667,166.667,-77.850,-77.850) Scott Base ENVELOPE(166.766,166.766,-77.849,-77.849) The Antarctic Environmental Microbiology 8 7 1177 1190 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary The community structure and composition of marine microbial biofilms established on glass surfaces was investigated across three differentially contaminated Antarctic sites within McMurdo Sound. Diverse microbial communities were revealed at all sites using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques. Sequencing of excised DGGE bands demonstrated close affiliation with known psychrophiles or undescribed bacteria also recovered from the Antarctic environment. The majority of bacterial sequences were affiliated to the Gammaproteobacteria , Cytophaga/Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia and Planctomycetales . Principal components analysis of quantitative FISH data revealed distinct differences in community composition between sites. Each of the sites were dominated by different bacterial groups: Alphaproteobacteria , Gammaproteobacteria and CFB at the least impacted site, Cape Armitage; green sulfur and sulfate reducing bacteria near the semi‐impacted Scott Base and Planctomycetales and sulfate reducing bacteria near the highly impacted McMurdo Station. The highest abundance of archaea was detected near Scott Base (2.5% of total bacteria). Multivariate analyses (non‐metric multidimensional scaling and analysis of similarities) of DGGE patterns revealed greater variability in community composition between sites than within sites. This is the first investigation of Antarctic biofilm structure and FISH results suggest that anthropogenic impacts may influence the complex composition of microbial communities. |
format |
Article in Journal/Newspaper |
author |
Webster, Nicole S. Negri, Andrew P. |
spellingShingle |
Webster, Nicole S. Negri, Andrew P. Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
author_facet |
Webster, Nicole S. Negri, Andrew P. |
author_sort |
Webster, Nicole S. |
title |
Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
title_short |
Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
title_full |
Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
title_fullStr |
Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
title_full_unstemmed |
Site‐specific variation in Antarctic marine biofilms established on artificial surfaces |
title_sort |
site‐specific variation in antarctic marine biofilms established on artificial surfaces |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1462-2920.2006.01007.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1462-2920.2006.01007.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1462-2920.2006.01007.x/fullpdf |
long_lat |
ENVELOPE(166.667,166.667,-77.850,-77.850) ENVELOPE(163.250,163.250,-78.150,-78.150) ENVELOPE(166.667,166.667,-77.850,-77.850) ENVELOPE(166.766,166.766,-77.849,-77.849) |
geographic |
Antarctic Armitage Cape Armitage McMurdo Sound McMurdo Station Scott Base The Antarctic |
geographic_facet |
Antarctic Armitage Cape Armitage McMurdo Sound McMurdo Station Scott Base The Antarctic |
genre |
Antarc* Antarctic McMurdo Sound |
genre_facet |
Antarc* Antarctic McMurdo Sound |
op_source |
Environmental Microbiology volume 8, issue 7, page 1177-1190 ISSN 1462-2912 1462-2920 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01007.x |
container_title |
Environmental Microbiology |
container_volume |
8 |
container_issue |
7 |
container_start_page |
1177 |
op_container_end_page |
1190 |
_version_ |
1802638068076773376 |