European Eel Sperm Diluent for Short‐term Storage
Contents The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining ov...
Published in: | Reproduction in Domestic Animals |
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Online Access: | http://dx.doi.org/10.1111/j.1439-0531.2008.01206.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0531.2008.01206.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0531.2008.01206.x |
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crwiley:10.1111/j.1439-0531.2008.01206.x 2024-06-02T08:06:15+00:00 European Eel Sperm Diluent for Short‐term Storage Peñaranda, DS Pérez, L Gallego, V Barrera, R Jover, M Asturiano, JF 2010 http://dx.doi.org/10.1111/j.1439-0531.2008.01206.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0531.2008.01206.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0531.2008.01206.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Reproduction in Domestic Animals volume 45, issue 3, page 407-415 ISSN 0936-6768 1439-0531 journal-article 2010 crwiley https://doi.org/10.1111/j.1439-0531.2008.01206.x 2024-05-03T10:36:07Z Contents The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining over 3 weeks’ conservation times under refrigeration, but they rendered bad results in the European species. In the present study, several diluents were developed taking as basis the P1 medium, and using different dilution ratios (1 : 50, 1 : 100) and two pH (6.5, 8.5). The effect of the addition of bovine serum albumin (BSA, 2% w/v) was also evaluated. At 24 h, undiluted samples already showed significant lower motility and viability than sperm samples diluted in the different media. The results for diluents with pH 6.5 and 8.5 were different. Spermatozoa diluted in media at pH 6.5 cannot be activated at 24 h, while samples diluted in the diluents with pH 8.5 and added with BSA did not show significant differences with respect to the fresh sperm motility until 48 h. The viability (percentage of alive cells) did not show differences until 1 week, independent of the dilution ratio. After 1 week, the motility was approximately 30% in the media containing BSA, which presented no differences for head size of the spermatozoa (perimeter and area) until 72 h and 1 week, respectively. In conclusion, the combination of one medium having similar physico‐chemical characteristics to the seminal plasma, including pH 8.5, and supplemented with BSA can be used in different dilution ratios for the sperm’s short‐term storage, preserving its motility capacity. Article in Journal/Newspaper European eel Wiley Online Library Reproduction in Domestic Animals 45 3 407 415 |
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English |
description |
Contents The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining over 3 weeks’ conservation times under refrigeration, but they rendered bad results in the European species. In the present study, several diluents were developed taking as basis the P1 medium, and using different dilution ratios (1 : 50, 1 : 100) and two pH (6.5, 8.5). The effect of the addition of bovine serum albumin (BSA, 2% w/v) was also evaluated. At 24 h, undiluted samples already showed significant lower motility and viability than sperm samples diluted in the different media. The results for diluents with pH 6.5 and 8.5 were different. Spermatozoa diluted in media at pH 6.5 cannot be activated at 24 h, while samples diluted in the diluents with pH 8.5 and added with BSA did not show significant differences with respect to the fresh sperm motility until 48 h. The viability (percentage of alive cells) did not show differences until 1 week, independent of the dilution ratio. After 1 week, the motility was approximately 30% in the media containing BSA, which presented no differences for head size of the spermatozoa (perimeter and area) until 72 h and 1 week, respectively. In conclusion, the combination of one medium having similar physico‐chemical characteristics to the seminal plasma, including pH 8.5, and supplemented with BSA can be used in different dilution ratios for the sperm’s short‐term storage, preserving its motility capacity. |
format |
Article in Journal/Newspaper |
author |
Peñaranda, DS Pérez, L Gallego, V Barrera, R Jover, M Asturiano, JF |
spellingShingle |
Peñaranda, DS Pérez, L Gallego, V Barrera, R Jover, M Asturiano, JF European Eel Sperm Diluent for Short‐term Storage |
author_facet |
Peñaranda, DS Pérez, L Gallego, V Barrera, R Jover, M Asturiano, JF |
author_sort |
Peñaranda, DS |
title |
European Eel Sperm Diluent for Short‐term Storage |
title_short |
European Eel Sperm Diluent for Short‐term Storage |
title_full |
European Eel Sperm Diluent for Short‐term Storage |
title_fullStr |
European Eel Sperm Diluent for Short‐term Storage |
title_full_unstemmed |
European Eel Sperm Diluent for Short‐term Storage |
title_sort |
european eel sperm diluent for short‐term storage |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1439-0531.2008.01206.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0531.2008.01206.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0531.2008.01206.x |
genre |
European eel |
genre_facet |
European eel |
op_source |
Reproduction in Domestic Animals volume 45, issue 3, page 407-415 ISSN 0936-6768 1439-0531 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1439-0531.2008.01206.x |
container_title |
Reproduction in Domestic Animals |
container_volume |
45 |
container_issue |
3 |
container_start_page |
407 |
op_container_end_page |
415 |
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1800751184852549632 |