Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae)
Contents The objective of this study was to optimize recovery and cryopreservation of epididymal sperm from plains bison, as a model for wood bison. In Phase 1, cauda epididymides were recovered from bison (n = 14) immediately after slaughter, minced and incubated in Sp‐TALPH buffer for 3 h at 36°C....
Published in: | Reproduction in Domestic Animals |
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crwiley:10.1111/j.1439-0531.2008.01087.x 2024-06-02T08:04:27+00:00 Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) Aurini, LC Whiteside, DP Elkin, BT Thundathil, JC 2009 http://dx.doi.org/10.1111/j.1439-0531.2008.01087.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0531.2008.01087.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0531.2008.01087.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Reproduction in Domestic Animals volume 44, issue 5, page 815-822 ISSN 0936-6768 1439-0531 journal-article 2009 crwiley https://doi.org/10.1111/j.1439-0531.2008.01087.x 2024-05-03T11:09:35Z Contents The objective of this study was to optimize recovery and cryopreservation of epididymal sperm from plains bison, as a model for wood bison. In Phase 1, cauda epididymides were recovered from bison (n = 14) immediately after slaughter, minced and incubated in Sp‐TALPH buffer for 3 h at 36°C. The resulting sperm suspensions were cryopreserved in Triladyl ® , using a protocol for bovine semen. In Phase 2, epididymal sperm were cryopreserved in either Triladyl ® or Andromed ® . The mean (±SD) estimated number of sperm recovered was 468 ± 207 × 10 6 . There was an increase (p < 0.05) in the proportion of sperm with normal morphology between initial recovery and after extension (52.4 ± 4.6 vs 69.7 ± 2.4%), with a concurrent decrease (p < 0.05) in the proportion of sperm with distal droplets. Median values for progressively motile sperm in post‐thaw samples (60%) were lower (p < 0.05) than that after extension or after chilling (70% for both). The mean percentages of viable sperm and of sperm with an intact acrosome were lower (p < 0.05) for frozen‐thawed samples (38.7 ± 2.8 and 85.2 ± 1.1) compared with extended (66.2 ± 2.2 and 92.4 ± 0.9) or chilled (63.7 ± 2.5 and 90.0 ± 1.0) samples. Rates of cleavage, morulae and blastocyst production were not significantly different for chilled (70.9, 38.7 and 8.0%) vs post‐thaw sperm (73.0, 46.0 and 6.3%). There was no significant difference between extenders for most sperm characteristics. In conclusion, we developed a functional protocol for the recovery and cryopreservation of epididymal sperm from plains bison, which may have implications for the genetic preservation of wood bison. Article in Journal/Newspaper Bison bison athabascae Wood Bison Bison bison bison Plains Bison Wiley Online Library Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Reproduction in Domestic Animals 44 5 815 822 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Contents The objective of this study was to optimize recovery and cryopreservation of epididymal sperm from plains bison, as a model for wood bison. In Phase 1, cauda epididymides were recovered from bison (n = 14) immediately after slaughter, minced and incubated in Sp‐TALPH buffer for 3 h at 36°C. The resulting sperm suspensions were cryopreserved in Triladyl ® , using a protocol for bovine semen. In Phase 2, epididymal sperm were cryopreserved in either Triladyl ® or Andromed ® . The mean (±SD) estimated number of sperm recovered was 468 ± 207 × 10 6 . There was an increase (p < 0.05) in the proportion of sperm with normal morphology between initial recovery and after extension (52.4 ± 4.6 vs 69.7 ± 2.4%), with a concurrent decrease (p < 0.05) in the proportion of sperm with distal droplets. Median values for progressively motile sperm in post‐thaw samples (60%) were lower (p < 0.05) than that after extension or after chilling (70% for both). The mean percentages of viable sperm and of sperm with an intact acrosome were lower (p < 0.05) for frozen‐thawed samples (38.7 ± 2.8 and 85.2 ± 1.1) compared with extended (66.2 ± 2.2 and 92.4 ± 0.9) or chilled (63.7 ± 2.5 and 90.0 ± 1.0) samples. Rates of cleavage, morulae and blastocyst production were not significantly different for chilled (70.9, 38.7 and 8.0%) vs post‐thaw sperm (73.0, 46.0 and 6.3%). There was no significant difference between extenders for most sperm characteristics. In conclusion, we developed a functional protocol for the recovery and cryopreservation of epididymal sperm from plains bison, which may have implications for the genetic preservation of wood bison. |
format |
Article in Journal/Newspaper |
author |
Aurini, LC Whiteside, DP Elkin, BT Thundathil, JC |
spellingShingle |
Aurini, LC Whiteside, DP Elkin, BT Thundathil, JC Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
author_facet |
Aurini, LC Whiteside, DP Elkin, BT Thundathil, JC |
author_sort |
Aurini, LC |
title |
Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
title_short |
Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
title_full |
Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
title_fullStr |
Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
title_full_unstemmed |
Recovery and Cryopreservation of Epididymal Sperm of Plains Bison ( Bison bison bison) as a Model for Salvaging the Genetics of Wood Bison ( Bison bison athabascae) |
title_sort |
recovery and cryopreservation of epididymal sperm of plains bison ( bison bison bison) as a model for salvaging the genetics of wood bison ( bison bison athabascae) |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1439-0531.2008.01087.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0531.2008.01087.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0531.2008.01087.x |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Slaughter |
geographic_facet |
Slaughter |
genre |
Bison bison athabascae Wood Bison Bison bison bison Plains Bison |
genre_facet |
Bison bison athabascae Wood Bison Bison bison bison Plains Bison |
op_source |
Reproduction in Domestic Animals volume 44, issue 5, page 815-822 ISSN 0936-6768 1439-0531 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1439-0531.2008.01087.x |
container_title |
Reproduction in Domestic Animals |
container_volume |
44 |
container_issue |
5 |
container_start_page |
815 |
op_container_end_page |
822 |
_version_ |
1800749081182601216 |