Violet Mink Develop an Acute Disease after Experimental Infection with Aleutian Disease Virus (ADV) Isolate ADV SL3

• Published in: Journal of Veterinary Medicine, Series B
• Main Authors: Haas, L., Wohlsein, P., Trautwein, G., Stolze, B., Kaaden, O.‐R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1990
• Subjects: Aleuten
• Online Access: http://dx.doi.org/10.1111/j.1439-0450.1990.tb01033.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1439-0450.1990.tb01033.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1439-0450.1990.tb01033.x

Summary Six‐Aleutian (aa)‐genotype violet mink were infected intraperitoneally with the Aleutian Disease Virus (ADV) bone marrow derived isolate ADV SL3. All animals developed virus‐specific antibodies and hypergammaglobulinaemia. Mortality during the fourteen week duration of the infection was 50%. The virus induced (histo)pathological lesions typical for Aleutian Disease. By immunohistochemical examination using a virus capsid‐specific monoclonal antibody viral antigen was detected in lymph nodes, spleen, kidneys and once in hepatic Kupffer cells. By Southern blot and in situ hybridization studies with strand‐specific RNA probes able to distinguish viral replicative forms from merely sequestered genomic DNA, ADV replication was detected in mesenteric lymph nodes and spleen. In one mink DNA replicative forms were also found in bone marrow cells or mononuclear cells of the peripheral blood, respectively. Only single‐stranded viral DNA was detected in liver, kidney, gut and lung of infected animals. From Southern blot hybridization results a different, possibly organ‐specific permissiveness of ADV in vivo is suggested. Zusammenfassung Akute Erkrankung von Aleuten‐Genotyp‐Nerzen nach experimenteller Infektion mit dem Aleutian Disease Virus (ADV)‐Isolat ADV SL3 Sechs Aleutian(aa)‐Genotyp‐Nerze wurden mit dem Aleutian Disease Virus (ADV)‐Knochen‐markisolat ADV SL3 intraperitoneal infiziert. Alle Tiere serokonvertierten und entwickelten eine Hypergammaglobulinämie. Die Mortalität innerhalb der 14wöchigen Versuchsdauer betrug 50%. Das Virus induzierte Aleutian Disease‐typische (histo)pathologische Alterationen. Aufgrund immunhistochemischer Untersuchungen mit einem Viruskapsid‐spezifischen monoklonalen Antikörper wurde virales Antigen in Lymphknoten, Milz und Nieren, sowie einmal in Kupfferschen Sternzellen der Leber gefunden. Mittels Southern blot‐ und in situ ‐Hybridisierungen mit strangspezifischen RNA‐Sonden, die eine Diskriminierung virusreplikativer von (sequestrierter) viraler Genom‐DNA erlaubten, konnte eine ...