Kinetic Evidence for the Existence of a Rate‐Limiting Step in the Reaction of Ferric Hemoproteins with Anionic Ligands

• Published in: European Journal of Biochemistry
• Main Authors: Coletta, Massimo, Angeletti, Mauro, De Sanctis, Giampiero, Cerroni, Loredana, Giardina, Bruno, Amiconi, Gino, Ascenzi, Paolo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1996
• Subjects: Dromedary; Sperm whale
• Online Access: http://dx.doi.org/10.1111/j.1432-1033.1996.00049.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1996.00049.x; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1996.00049.x

The kinetics of azide and fluoride binding to various monomeric and tetrameric ferric hemoproteins (sperm whale Mb, isolated α and β chains of human Hb reacted with p ‐chloromercuribenzoate, dromedary, ox and human Hb) has been investigated (at pH 6.5 and 20°C) over a large range (20 μM to 2 M) of l...