Improvement of anther culture technique: Activated charcoal bound in agar medium in combination with liquid medium and elevated CO 2 concentration

• Published in: Physiologia Plantarum
• Main Authors: Johansson, Lars, Andersson, Barbro, Eriksson, Tage
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1982
• Subjects: Gibba; Papaver radicatum
• Online Access: http://dx.doi.org/10.1111/j.1399-3054.1982.tb00571.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-3054.1982.tb00571.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1982.tb00571.x

Anthers of different species of the genera Anemone, Clematis, Papaver and Nicotiana were cultured by floating on a liquid medium which overlay an agarified charcoal medium . This technique proved to be superior to conventional methods i.e. culture on either solid or liquid media. Cold treatment of A...